Metabolic cross-feeding via intercellular nanotubes among bacteria

Samay Pande, Shraddha Shitut, Lisa Freund, Martin Westermann, Felix Bertels, Claudia Colesie, Ilka B. Bischofs, Christian Kost*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Bacteria frequently exchange metabolites by diffusion through the extracellular environment, yet it remains generally unclear whether bacteria can also use cell-cell connections to directly exchange nutrients. Here we address this question by engineering cross-feeding interactions within and between Acinetobacter baylyi and Escherichia coli, in which two distant bacterial species reciprocally exchange essential amino acids. We establish that in a well-mixed environment E. coli, but likely not A. baylyi, can connect to other bacterial cells via membrane-derived nanotubes and use these to exchange cytoplasmic constituents. Intercellular connections are induced by auxotrophy-causing mutations and cease to establish when amino acids are externally supplied. Electron and fluorescence microscopy reveal a network of nanotubular structures that connects bacterial cells and enables an intercellular transfer of cytoplasmic materials. Together, our results demonstrate that bacteria can use nanotubes to exchange nutrients among connected cells and thus help to distribute metabolic functions within microbial communities.

Original languageEnglish
Article number6238
JournalNature Communications
Volume6
DOIs
Publication statusPublished - 23 Feb 2015

Fingerprint

Dive into the research topics of 'Metabolic cross-feeding via intercellular nanotubes among bacteria'. Together they form a unique fingerprint.

Cite this