Metabolism of dietary (Acetyl-1-14c)-plant cell walls in the rat intestine

C J BUCHANAN, M A EASTWOOD, Stephen Fry

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Abstract

To determine a fate of acetyl groups in intact dietary plant cell walls (PCW) spinach cells were labelled using [1-C-14]acetate as a precursor and the PCW isolated. The (acetyl-C-14)-PCW were fed to rats by gavage and the C-14 traced in the rat gut and in the body tissues and excreta of the rat. Some label was solubilised during passage through the upper intestine of the rat, and subsequently incorporated into the body tissues of the rat. A small amount of the radioactivity was excreted as CO2 (4%) and in urine (<1%). (Acetyl-C-14)-PCW which reached the caecum was solubilised and some [C-14]acetate released. The solubilised C-14 was incorporated in liver tissue mainly as amino acid present in protein.

Original languageEnglish
Pages (from-to)367-374
Number of pages8
JournalJournal of the Science of Food and Agriculture
Volume67
Issue number3
DOIs
Publication statusPublished - Mar 1995

Keywords

  • plant cell wall
  • fermentation
  • acetate

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