Metal binding to a zinc-finger peptide: a comparison between solution and the gas phase

Yana Berezovskaya; Craig T. Armstrong; Aimee L. Boyle; Massimiliano Porrini; Derek N. Woolfson; Perdita E. Barran

doi:10.1039/c0cc02445g

Metal binding to a zinc-finger peptide: a comparison between solution and the gas phase

Yana Berezovskaya, Craig T. Armstrong, Aimee L. Boyle, Massimiliano Porrini, Derek N. Woolfson, Perdita E. Barran

Research output: Contribution to journal › Article › peer-review

Abstract

Solution-phase spectroscopy and mass spectrometry are used to probe interactions between divalent metal ions and a synthetic Cys(2)His(2) zinc-finger peptide (vCP1). Both methods provide the same order of binding affinity, zinc >= cobalt >> copper >> calcium. Collision-cross-section measurements show that both apo and holo forms are compact. This is corroborated by molecular-dynamics simulations.

Original language	English
Pages (from-to)	412-414
Number of pages	3
Journal	Chemical Communications
Volume	47
Issue number	1
DOIs	https://doi.org/10.1039/c0cc02445g
Publication status	Published - 2011

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10.1039/c0cc02445g

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title = "Metal binding to a zinc-finger peptide: a comparison between solution and the gas phase",

abstract = "Solution-phase spectroscopy and mass spectrometry are used to probe interactions between divalent metal ions and a synthetic Cys(2)His(2) zinc-finger peptide (vCP1). Both methods provide the same order of binding affinity, zinc >= cobalt >> copper >> calcium. Collision-cross-section measurements show that both apo and holo forms are compact. This is corroborated by molecular-dynamics simulations.",

author = "Yana Berezovskaya and Armstrong, {Craig T.} and Boyle, {Aimee L.} and Massimiliano Porrini and Woolfson, {Derek N.} and Barran, {Perdita E.}",

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AU - Porrini, Massimiliano

AU - Woolfson, Derek N.

AU - Barran, Perdita E.

PY - 2011

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AB - Solution-phase spectroscopy and mass spectrometry are used to probe interactions between divalent metal ions and a synthetic Cys(2)His(2) zinc-finger peptide (vCP1). Both methods provide the same order of binding affinity, zinc >= cobalt >> copper >> calcium. Collision-cross-section measurements show that both apo and holo forms are compact. This is corroborated by molecular-dynamics simulations.

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DO - 10.1039/c0cc02445g

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EP - 414

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

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Metal binding to a zinc-finger peptide: a comparison between solution and the gas phase

Abstract

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The MoQToF: Development of an Ion Mobility Mass Spectrometer to Study Structure and Energetics of Proteins and Peptides

ARF: Structure and energetics of peptides and small proteins

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Metal binding to a zinc-finger peptide: a comparison between solution and the gas phase

Abstract

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Projects

The MoQToF: Development of an Ion Mobility Mass Spectrometer to Study Structure and Energetics of Proteins and Peptides

ARF: Structure and energetics of peptides and small proteins

Cite this