Abstract
Background. Staphylococcus pseudintermedius is a common opportunistic pathogen of companion dogs and an occasional human pathogen. Treatment is hampered by antimicrobial resistance including methicillin-resistance encoded by mecA within the mobile genetic element SCCmec.
Objectives. SCCmec elements are diverse, especially in non-Staphyloccocus aureus staphylococci, and novel variants are likely to be present in S. pseudintermedius. The aim was to characterise the SCCmec elements found in four canine clinical isolates of S. pseudintermedius.
Methods. Isolates were whole-genome sequenced and SCCmec elements were assembled, annotated and compared to known SCCmec types.
Results. Two novel SSCmec are present in these isolates. SCCmec7017-61515 is characterised by a novel combination of a Class A mec gene complex and a type 5 ccr previously only described in composite SCCmec elements. The other three isolates share a novel composite SCCmec with features of SCCmec types IV and VI.
Conclusions. S. pseudintermedius is a reservoir of novel SSCmec elements which has implications for understanding antimicrobial resistant in veterinary and human medicine.
Objectives. SCCmec elements are diverse, especially in non-Staphyloccocus aureus staphylococci, and novel variants are likely to be present in S. pseudintermedius. The aim was to characterise the SCCmec elements found in four canine clinical isolates of S. pseudintermedius.
Methods. Isolates were whole-genome sequenced and SCCmec elements were assembled, annotated and compared to known SCCmec types.
Results. Two novel SSCmec are present in these isolates. SCCmec7017-61515 is characterised by a novel combination of a Class A mec gene complex and a type 5 ccr previously only described in composite SCCmec elements. The other three isolates share a novel composite SCCmec with features of SCCmec types IV and VI.
Conclusions. S. pseudintermedius is a reservoir of novel SSCmec elements which has implications for understanding antimicrobial resistant in veterinary and human medicine.
| Original language | English |
|---|---|
| Pages (from-to) | 1303-1308 |
| Number of pages | 6 |
| Journal | Journal of Antimicrobial Chemotherapy |
| Volume | 79 |
| Issue number | 6 |
| Early online date | 2 Apr 2024 |
| DOIs | |
| Publication status | Published - 1 Jun 2024 |
Keywords / Materials (for Non-textual outputs)
- Animals
- Anti-Bacterial Agents/pharmacology
- Bacterial Proteins/genetics
- Chromosomes, Bacterial/genetics
- Dog Diseases/microbiology
- Dogs
- Genetic Variation
- Genome, Bacterial
- Interspersed Repetitive Sequences/genetics
- Methicillin Resistance/genetics
- Microbial Sensitivity Tests
- Staphylococcal Infections/microbiology
- Staphylococcus/genetics
- Whole Genome Sequencing