Methods for Ex Vivo Analysis of Immune Cell Function from the Central Nervous System

Darryl G Turner, Melanie D Leech, Richard A O'Connor, Stephen M Anderton

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Experimental autoimmune encephalomyelitis (EAE) is an animal model commonly used to investigate the inflammatory response in organ-specific autoimmunity and a model of the early immune responses of multiple sclerosis.This protocol outlines the methods used for the processing of peripheral immune tissues, the spleen and draining lymph nodes, as well as the site of inflammation, the central nervous system (CNS), for analyzing immune cell phenotype and function during murine EAE.

Original languageEnglish
Title of host publicationMultiple Sclerosis
Subtitle of host publicationMethods and Protocols
PublisherSpringerLink
Pages81-90
Number of pages10
Volume1304
ISBN (Electronic)978-1-4939-2630-5
ISBN (Print)978-1-4939-2629-9
DOIs
Publication statusPublished - Apr 2015

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