Methods for Ex Vivo Analysis of Immune Cell Function from the Central Nervous System

Darryl G Turner; Melanie D Leech; Richard A O'Connor; Stephen M Anderton

doi:10.1007/7651_2015_222

Methods for Ex Vivo Analysis of Immune Cell Function from the Central Nervous System

Darryl G Turner, Melanie D Leech, Richard A O'Connor, Stephen M Anderton

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Experimental autoimmune encephalomyelitis (EAE) is an animal model commonly used to investigate the inflammatory response in organ-specific autoimmunity and a model of the early immune responses of multiple sclerosis.This protocol outlines the methods used for the processing of peripheral immune tissues, the spleen and draining lymph nodes, as well as the site of inflammation, the central nervous system (CNS), for analyzing immune cell phenotype and function during murine EAE.

Original language	English
Title of host publication	Multiple Sclerosis
Subtitle of host publication	Methods and Protocols
Publisher	SpringerLink
Pages	81-90
Number of pages	10
Volume	1304
ISBN (Electronic)	978-1-4939-2630-5
ISBN (Print)	978-1-4939-2629-9
DOIs	https://doi.org/10.1007/7651_2015_222
Publication status	Published - Apr 2015

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title = "Methods for Ex Vivo Analysis of Immune Cell Function from the Central Nervous System",

abstract = "Experimental autoimmune encephalomyelitis (EAE) is an animal model commonly used to investigate the inflammatory response in organ-specific autoimmunity and a model of the early immune responses of multiple sclerosis.This protocol outlines the methods used for the processing of peripheral immune tissues, the spleen and draining lymph nodes, as well as the site of inflammation, the central nervous system (CNS), for analyzing immune cell phenotype and function during murine EAE.",

author = "Turner, {Darryl G} and Leech, {Melanie D} and O'Connor, {Richard A} and Anderton, {Stephen M}",

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AU - Leech, Melanie D

AU - O'Connor, Richard A

AU - Anderton, Stephen M

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AB - Experimental autoimmune encephalomyelitis (EAE) is an animal model commonly used to investigate the inflammatory response in organ-specific autoimmunity and a model of the early immune responses of multiple sclerosis.This protocol outlines the methods used for the processing of peripheral immune tissues, the spleen and draining lymph nodes, as well as the site of inflammation, the central nervous system (CNS), for analyzing immune cell phenotype and function during murine EAE.

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DO - 10.1007/7651_2015_222

M3 - Chapter

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SN - 978-1-4939-2629-9

VL - 1304

SP - 81

EP - 90

BT - Multiple Sclerosis

PB - SpringerLink

ER -

Methods for Ex Vivo Analysis of Immune Cell Function from the Central Nervous System

Abstract

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