Methods for Precisely Localised Transfer of Cells or DNA into Early Postimplantation Mouse Embryos

Yali Huang; Ron Wilkie; Valerie Wilson

doi:10.3791/53295

Methods for Precisely Localised Transfer of Cells or DNA into Early Postimplantation Mouse Embryos

Yali Huang, Ron Wilkie, Valerie Wilson

Research output: Contribution to journal › Article › peer-review

Abstract

We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the precise delivery of exogenous DNA into a few cells in the embryos.

Original language	English
Article number	e53295
Journal	Journal of Visualized Experiments (JoVE)
Issue number	106
DOIs	https://doi.org/10.3791/53295
Publication status	Published - 25 Dec 2015

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10.3791/53295

Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos
Copyright © 2015 Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License
Final published version, 851 KBLicence: Creative Commons: Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND)

Specification, maintenance and elimination of stem cell progenitors for the mammalian anteroposterior axis
Wilson, V. (Principal Investigator)
MRC
1/01/13 → 31/12/18
Project: Research

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title = "Methods for Precisely Localised Transfer of Cells or DNA into Early Postimplantation Mouse Embryos",

abstract = "We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the precise delivery of exogenous DNA into a few cells in the embryos.",

author = "Yali Huang and Ron Wilkie and Valerie Wilson",

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AU - Huang, Yali

AU - Wilkie, Ron

AU - Wilson, Valerie

N1 - No MS or accepted publication date/email supplied by author. Paper published 18 January (2016).

PY - 2015/12/25

Y1 - 2015/12/25

N2 - We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the precise delivery of exogenous DNA into a few cells in the embryos.

AB - We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the precise delivery of exogenous DNA into a few cells in the embryos.

U2 - 10.3791/53295

DO - 10.3791/53295

M3 - Article

SN - 1940-087X

JO - Journal of Visualized Experiments (JoVE)

JF - Journal of Visualized Experiments (JoVE)

IS - 106

M1 - e53295

ER -

Methods for Precisely Localised Transfer of Cells or DNA into Early Postimplantation Mouse Embryos

Abstract

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Specification, maintenance and elimination of stem cell progenitors for the mammalian anteroposterior axis

Cite this