Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome

D K Griffin; F Haberman; J Masabanda; P O'Brien; M Bagga; A Sazanov; Jacqueline Smith; D W Burt; M Ferguson-Smith; J Wienberg

doi:10.1159/000015449

Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome

D K Griffin, F Haberman, J Masabanda, P O'Brien, M Bagga, A Sazanov, Jacqueline Smith, D W Burt, M Ferguson-Smith, J Wienberg

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

Despite the chicken being one of the most genetically mapped of all animals, its karyotype remains poorly defined. This is primarily due to microchromosomes that belie assignment by conventional methods. To address this problem, we have developed chromosome-specific paints using flow cytometry and microdissection. For the microchromosomes it was necessary to amplify and label DNA from single microdissected chromosomes.

Original language	English
Pages (from-to)	278-81
Number of pages	4
Journal	Cytogenetics and Cell Genetics
Volume	87
Issue number	3-4
DOIs	https://doi.org/10.1159/000015449
Publication status	Published - 1999

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title = "Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome",

abstract = "Despite the chicken being one of the most genetically mapped of all animals, its karyotype remains poorly defined. This is primarily due to microchromosomes that belie assignment by conventional methods. To address this problem, we have developed chromosome-specific paints using flow cytometry and microdissection. For the microchromosomes it was necessary to amplify and label DNA from single microdissected chromosomes.",

author = "Griffin, {D K} and F Haberman and J Masabanda and P O'Brien and M Bagga and A Sazanov and Jacqueline Smith and Burt, {D W} and M Ferguson-Smith and J Wienberg",

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T2 - tools for mapping the chicken genome

AU - Griffin, D K

AU - Haberman, F

AU - Masabanda, J

AU - O'Brien, P

AU - Bagga, M

AU - Sazanov, A

AU - Smith, Jacqueline

AU - Burt, D W

AU - Ferguson-Smith, M

AU - Wienberg, J

PY - 1999

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N2 - Despite the chicken being one of the most genetically mapped of all animals, its karyotype remains poorly defined. This is primarily due to microchromosomes that belie assignment by conventional methods. To address this problem, we have developed chromosome-specific paints using flow cytometry and microdissection. For the microchromosomes it was necessary to amplify and label DNA from single microdissected chromosomes.

AB - Despite the chicken being one of the most genetically mapped of all animals, its karyotype remains poorly defined. This is primarily due to microchromosomes that belie assignment by conventional methods. To address this problem, we have developed chromosome-specific paints using flow cytometry and microdissection. For the microchromosomes it was necessary to amplify and label DNA from single microdissected chromosomes.

U2 - 10.1159/000015449

DO - 10.1159/000015449

M3 - Article

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SN - 0301-0171

VL - 87

SP - 278

EP - 281

JO - Cytogenetics and Cell Genetics

JF - Cytogenetics and Cell Genetics

IS - 3-4

ER -

Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome

Abstract

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