Microarray profiling emphasizes transcriptomic differences between hippocampal in vivo tissue and in vitro cultures" for publication in Brain Communications.

Declan King, Paul Skehel, Owen Dando, Katie Emelianova, Rona Barron, Thomas Wishart

Research output: Contribution to journalArticlepeer-review

Abstract

Primary hippocampal cell cultures are routinely used as an experimentally accessible model platform for the hippocampus and brain tissue in general. Containing multiple cell types including neurons, astrocytes and microglia in a state that can be readily analysed optically, biochemically and electrophysiologically, such cultures have been used in many in vitro studies. To what extent the in vivo environment is recapitulated in primary cultures in an ongoing question. Here we compare the transcriptomic profiles of primary hippocampal cell cultures and intact hippocampal tissue. In addition, by comparing profiles from wild type and the PrP 101LL transgenic model of prion disease, we also demonstrate that gene conservation is predominantly conserved across genetically altered lines.
Original languageEnglish
Article numberfcab152
JournalBrain Communications
Volume3
Issue number3
Early online date7 Jul 2021
DOIs
Publication statusE-pub ahead of print - 7 Jul 2021

Keywords / Materials (for Non-textual outputs)

  • Hippocampal cultures
  • transcriptome
  • microarray,
  • neuronal.

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