Microbial community structure changes during bioremediation of PAHs in an aged coal-tar contaminated soil by in-vessel composting

Blanca Antizar Ladislao, Katerina Spanova, Angus J. Beck, Nicholas J. Russell

Research output: Contribution to journalArticlepeer-review

Abstract

The microbial community structure changes of an aged-coal-tar soil contaminated with polycyclic aromatic hydrocarbons (PAHs) were investigated during simulated bioremediation at the laboratory-scale using an in-vessel composting approach. The composting reactors were operated using a logistic three-factor factorial design with three temperatures (T = 38, 55 or 70 degrees C), four soil to green-waste amendment ratios (S:GW = 0.6:1, 0.7:1, 0.8:1 or 0.9:1 on a dry weight basis) and three moisture contents (MC = 40%, 60% or 80%). Relative changes in microbial populations were investigated by following the dynamics of phospholipid fatty acid (PLFA) signatures using a C-13-labeled palmitic acid internal standard and sensitive GC/MS analysis during in-vessel composting over 98 days. The results of this investigation indicated that fungal to bacterial PLFA ratios were significantly influenced by temperature (p<0.05), and Gram-positive to Gram-negative bacterial ratios were significantly influenced by temperature (p<0.001) and S:GW ratio (p<0.01) during in-vessel composting. Additionally, the Gram-positive to Gram-negative bacterial ratios were correlated to the extent of PAH losses)<0.005) at 70 degrees C. (C) 2007 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)357-364
Number of pages8
JournalInternational Biodeterioration and Biodegradation
Volume61
Issue number4
DOIs
Publication statusPublished - Jun 2008

Keywords

  • bioremediation
  • coal-tar
  • soil
  • composting
  • polycyclic aromatic hydrocarbons
  • phospholipid fatty acids
  • POLYCYCLIC AROMATIC-HYDROCARBONS
  • PHOSPHOLIPID FATTY-ACIDS
  • WHITE-ROT FUNGI
  • SEWAGE-SLUDGE
  • BIODEGRADATION
  • DEGRADATION
  • TEMPERATURE
  • REMEDIATION
  • MICROFLORA
  • BACTERIAL

Cite this