Microplate technique to determine hemolytic activity for routine typing of Listeria strains

L Dominguez Rodriguez, Jose A Vazquez-Boland, Jose Francisco Fernández-Garayzábal, P Echalecu Tranchant, E Gomez-Lucia, Elias Fernando Rodriguez-Ferri

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Because the hemolysis produced by Listeria monocytogenes and Listeria seeligeri on blood agar is frequently difficult to interpret, we developed a microplate technique for the routine determination of hemolytic activity with erythrocyte suspensions. This microtechnique is a simple and reliable test for distinguishing clearly between hemolytic and nonhemolytic strains and could be used instead of the CAMP (Christie-Atkins-Munch-Petersen) test with Staphylococcus aureus in the routine typing of Listeria strains. Furthermore, our results suggest that the quantitation of the hemolytic activity of the Listeria strains, along with the D-xylose, L-rhamnose, and alpha-methyl-D-mannoside acidification tests, allows the differentiation of L. monocytogenes, L. seeligeri, and Listeria ivanovii. We also observed that the treatment of erythrocytes with crude exosubstances of rhodococcus equi, Pseudomonas fluorescens, Acinetobacter calcoaceticus, and S. aureus enhanced the hemolytic activity of all Listeria strains with this characteristic.
Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalJournal of Clinical Microbiology
Issue number1
Publication statusPublished - Jul 1986


  • Acinetobacter/physiology
  • Actinomycetales/physiology
  • Bacteriological Techniques
  • Hemolysis
  • Listeria/classification
  • Listeria/physiology
  • Listeria monocytogenes/classification
  • Listeria monocytogenes/physiology
  • Pseudomonas fluorescens/physiology
  • Staphylococcus aureus/physiology


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