MicroRNA-143 Activation Regulates Smooth Muscle and Endothelial Cell Crosstalk in Pulmonary Arterial Hypertension

Lin Deng, Francisco J Blanco, Hannah Stevens, Ruifang Lu, Axelle Caudrillier, Martin McBride, John D McClure, Jenny Grant, Matthew Thomas, Maria Frid, Kurt Stenmark, Kevin White, Anita G Seto, Nicholas W Morrell, Angela C Bradshaw, Margaret R MacLean, Andrew H Baker

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

RATIONALE: The pathogenesis of pulmonary arterial hypertension (PAH) remains unclear. The 4 microRNAs representing the miR-143 and miR-145 stem loops are genomically clustered.

OBJECTIVE: To elucidate the transcriptional regulation of the miR-143/145 cluster and the role of miR-143 in PAH.

METHODS AND RESULTS: We identified the promoter region that regulates miR-143/145 microRNA expression in pulmonary artery smooth muscle cells (PASMCs). We mapped PAH-related signaling pathways, including estrogen receptor, liver X factor/retinoic X receptor, transforming growth factor-β (Smads), and hypoxia (hypoxia response element), that regulated levels of all pri-miR stem loop transcription and resulting microRNA expression. We observed that miR-143-3p is selectively upregulated compared with miR-143-5p during PASMC migration. Modulation of miR-143 in PASMCs significantly altered cell migration and apoptosis. In addition, we found high abundance of miR-143-3p in PASMC-derived exosomes. Using assays with pulmonary arterial endothelial cells, we demonstrated a paracrine promigratory and proangiogenic effect of miR-143-3p-enriched exosomes from PASMC. Quantitative polymerase chain reaction and in situ hybridization showed elevated expression of miR-143 in calf models of PAH and in samples from PAH patients. Moreover, in contrast to our previous findings that had not supported a therapeutic role in vivo, we now demonstrate a protective role of miR-143 in experimental pulmonary hypertension in vivo in miR-143-/- and anti-miR-143-3p-treated mice exposed to chronic hypoxia in both preventative and reversal settings.

CONCLUSIONS: MiR-143-3p modulated both cellular and exosome-mediated responses in pulmonary vascular cells, whereas inhibition of miR-143-3p blocked experimental pulmonary hypertension. Taken together, these findings confirm an important role for the miR-143/145 cluster in PAH pathobiology.

Original languageEnglish
Pages (from-to)870-883
Number of pages14
JournalCirculation Research
Issue number10
Early online date26 Aug 2015
Publication statusPublished - 23 Oct 2015

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Arterial Pressure
  • Binding Sites
  • Case-Control Studies
  • Cattle
  • Cell Communication
  • Cell Movement
  • Endothelial Cells
  • Exosomes
  • Female
  • Gene Expression Regulation
  • HeLa Cells
  • Humans
  • Hypertension, Pulmonary
  • Male
  • Mice, Inbred C57BL
  • Mice, Knockout
  • MicroRNAs
  • Muscle, Smooth, Vascular
  • Myocytes, Smooth Muscle
  • Promoter Regions, Genetic
  • Pulmonary Artery
  • Signal Transduction
  • Time Factors
  • Transcription Factors
  • Transfection
  • Vascular Remodeling
  • Ventricular Function, Right
  • Ventricular Pressure


Dive into the research topics of 'MicroRNA-143 Activation Regulates Smooth Muscle and Endothelial Cell Crosstalk in Pulmonary Arterial Hypertension'. Together they form a unique fingerprint.

Cite this