MicroRNA-92b regulates the development of intermediate cortical progenitors in embryonic mouse brain

Tomasz Jan Nowakowski, Vassiliki Fotaki, Andrew Pollock, Tao Sun, Thomas Pratt, David J Price

Research output: Contribution to journalArticlepeer-review

Abstract

Cerebral cortical neurons arise from radial glia (direct neurogenesis) or from intermediate progenitors (indirect neurogenesis); intriguingly, the sizes of intermediate progenitor populations and the cortices they generate correlate across species. The generation of intermediate progenitors is regulated by the transcription factor Tbr2, whose expression marks these cells. We investigated how this mechanism might be controlled. We found that acute blockade of mature microRNA biosynthesis in murine cortical progenitors caused a rapid cell autonomous increase in numbers of Tbr2-expressing cells. Acute microRNA-92b (miR-92b) gain of function caused rapid reductions in numbers of Tbr2-expressing cells and proliferating intermediate progenitors. Acute miR-92b loss of function had opposite effects. These findings indicate that miR-92b limits the production of intermediate cortical progenitors.
Original languageEnglish
Pages (from-to)7056–7061
JournalProceedings of the National Academy of Sciences
Volume110
Issue number17
DOIs
Publication statusPublished - Apr 2013

Keywords

  • cortical development
  • microRNAs
  • Animals
  • Bromodeoxyuridine
  • Immunohistochemistry
  • MICE
  • MICRORNAS
  • Neural Stem Cells
  • NEUROGENESIS
  • NEURONS
  • Real-Time Polymerase Chain Reaction
  • T-Box Domain Proteins

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