Microsatellite genotyping of medieval cattle from central Italy suggests an old origin of Chianina and Romagnola cattle

Maria Gargani; Lorraine Pariset; Johannes Arjen Lenstra; Elisabetta De Minicis; K. Moazami-Goudarzi; D. Laloë; J. L. Williams; P. Wiener; D. G. Bradley; G. Erhardt; B. Harlizius; I. Medugorac; Munich C. Looft; E. Kalm; J. Cañón; S. Dunner; C. Rodellar; I. Martín-Burriel; A. Sanchez; A. M. Martínez; C. Ginja; P. Ajmone-Marsan; F. Pilla; A. Bruzzone; D. Marletta; L. E. Holm; G. Dolf; Alessio Valentini

doi:10.3389/fgene.2015.00068

Microsatellite genotyping of medieval cattle from central Italy suggests an old origin of Chianina and Romagnola cattle

Maria Gargani^*, Lorraine Pariset, Johannes Arjen Lenstra, Elisabetta De Minicis, K. Moazami-Goudarzi, D. Laloë, J. L. Williams, P. Wiener, D. G. Bradley, G. Erhardt, B. Harlizius, I. Medugorac, Munich C. Looft, E. Kalm, J. Cañón, S. Dunner, C. Rodellar, I. Martín-Burriel, A. Sanchez, A. M. MartínezC. Ginja, P. Ajmone-Marsan, F. Pilla, A. Bruzzone, D. Marletta, L. E. Holm, G. Dolf, Alessio Valentini

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Analysis of DNA from archaeological remains is a valuable tool to interpret the history of ancient animal populations. So far most studies of ancient DNA target mitochondrial DNA (mtDNA), which reveals maternal lineages, but only partially the relationships of current breeds and ancient populations. In this study we explore the feasibility of nuclear DNA analysis. DNA was extracted from 1000-years old cattle bone collected from Ferento, an archaeological site in central Italy. Amplification of 15 microsatellite FAO-recommended markers with PCR products yielded genotypes for four markers. Expected heterozygosity was comparable with values of modern breeds, but observed heterozygosity was underestimated due to allelic loss. Genetic distances suggested a position intermediate between (1) Anatolian, Balkan, Sicilian and South-Italian cattle and (2) the Iberian, North-European and Central-European cattle, but also a clear relationship with two central-Italian breeds, Chianina and Romagnola. This suggests that these breeds are derived from medieval cattle living in the same area. Our results illustrate the potential of ancient DNA for reconstructing the history of local cattle husbandry.

Original language	English
Article number	68
Journal	Frontiers in genetics
Volume	5
Issue number	FEB
DOIs	https://doi.org/10.3389/fgene.2015.00068
Publication status	Published - 1 Jan 2015

Keywords

Ancient DNA
Cattle
Ferento
Microsatellite
Neighbornet

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Gargani, M., Pariset, L., Lenstra, J. A., De Minicis, E., Moazami-Goudarzi, K., Laloë, D., Williams, J. L., Wiener, P., Bradley, D. G., Erhardt, G., Harlizius, B., Medugorac, I., Looft, M. C., Kalm, E., Cañón, J., Dunner, S., Rodellar, C., Martín-Burriel, I., Sanchez, A., ... Valentini, A. (2015). Microsatellite genotyping of medieval cattle from central Italy suggests an old origin of Chianina and Romagnola cattle. Frontiers in genetics, 5(FEB), [68]. https://doi.org/10.3389/fgene.2015.00068

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title = "Microsatellite genotyping of medieval cattle from central Italy suggests an old origin of Chianina and Romagnola cattle",

abstract = "Analysis of DNA from archaeological remains is a valuable tool to interpret the history of ancient animal populations. So far most studies of ancient DNA target mitochondrial DNA (mtDNA), which reveals maternal lineages, but only partially the relationships of current breeds and ancient populations. In this study we explore the feasibility of nuclear DNA analysis. DNA was extracted from 1000-years old cattle bone collected from Ferento, an archaeological site in central Italy. Amplification of 15 microsatellite FAO-recommended markers with PCR products yielded genotypes for four markers. Expected heterozygosity was comparable with values of modern breeds, but observed heterozygosity was underestimated due to allelic loss. Genetic distances suggested a position intermediate between (1) Anatolian, Balkan, Sicilian and South-Italian cattle and (2) the Iberian, North-European and Central-European cattle, but also a clear relationship with two central-Italian breeds, Chianina and Romagnola. This suggests that these breeds are derived from medieval cattle living in the same area. Our results illustrate the potential of ancient DNA for reconstructing the history of local cattle husbandry.",

keywords = "Ancient DNA, Cattle, Ferento, Microsatellite, Neighbornet",

author = "Maria Gargani and Lorraine Pariset and Lenstra, {Johannes Arjen} and {De Minicis}, Elisabetta and K. Moazami-Goudarzi and D. Lalo{\"e} and Williams, {J. L.} and P. Wiener and Bradley, {D. G.} and G. Erhardt and B. Harlizius and I. Medugorac and Looft, {Munich C.} and E. Kalm and J. Ca{\~n}{\'o}n and S. Dunner and C. Rodellar and I. Mart{\'i}n-Burriel and A. Sanchez and Mart{\'i}nez, {A. M.} and C. Ginja and P. Ajmone-Marsan and F. Pilla and A. Bruzzone and D. Marletta and Holm, {L. E.} and G. Dolf and Alessio Valentini",

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Gargani, M, Pariset, L, Lenstra, JA, De Minicis, E, Moazami-Goudarzi, K, Laloë, D, Williams, JL, Wiener, P, Bradley, DG, Erhardt, G, Harlizius, B, Medugorac, I, Looft, MC, Kalm, E, Cañón, J, Dunner, S, Rodellar, C, Martín-Burriel, I, Sanchez, A, Martínez, AM, Ginja, C, Ajmone-Marsan, P, Pilla, F, Bruzzone, A, Marletta, D, Holm, LE, Dolf, G & Valentini, A 2015, 'Microsatellite genotyping of medieval cattle from central Italy suggests an old origin of Chianina and Romagnola cattle', Frontiers in genetics, vol. 5, no. FEB, 68. https://doi.org/10.3389/fgene.2015.00068

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AU - Gargani, Maria

AU - Pariset, Lorraine

AU - Lenstra, Johannes Arjen

AU - De Minicis, Elisabetta

AU - Moazami-Goudarzi, K.

AU - Laloë, D.

AU - Williams, J. L.

AU - Wiener, P.

AU - Bradley, D. G.

AU - Erhardt, G.

AU - Harlizius, B.

AU - Medugorac, I.

AU - Looft, Munich C.

AU - Kalm, E.

AU - Cañón, J.

AU - Dunner, S.

AU - Rodellar, C.

AU - Martín-Burriel, I.

AU - Sanchez, A.

AU - Martínez, A. M.

AU - Ginja, C.

AU - Ajmone-Marsan, P.

AU - Pilla, F.

AU - Bruzzone, A.

AU - Marletta, D.

AU - Holm, L. E.

AU - Dolf, G.

AU - Valentini, Alessio

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Analysis of DNA from archaeological remains is a valuable tool to interpret the history of ancient animal populations. So far most studies of ancient DNA target mitochondrial DNA (mtDNA), which reveals maternal lineages, but only partially the relationships of current breeds and ancient populations. In this study we explore the feasibility of nuclear DNA analysis. DNA was extracted from 1000-years old cattle bone collected from Ferento, an archaeological site in central Italy. Amplification of 15 microsatellite FAO-recommended markers with PCR products yielded genotypes for four markers. Expected heterozygosity was comparable with values of modern breeds, but observed heterozygosity was underestimated due to allelic loss. Genetic distances suggested a position intermediate between (1) Anatolian, Balkan, Sicilian and South-Italian cattle and (2) the Iberian, North-European and Central-European cattle, but also a clear relationship with two central-Italian breeds, Chianina and Romagnola. This suggests that these breeds are derived from medieval cattle living in the same area. Our results illustrate the potential of ancient DNA for reconstructing the history of local cattle husbandry.

AB - Analysis of DNA from archaeological remains is a valuable tool to interpret the history of ancient animal populations. So far most studies of ancient DNA target mitochondrial DNA (mtDNA), which reveals maternal lineages, but only partially the relationships of current breeds and ancient populations. In this study we explore the feasibility of nuclear DNA analysis. DNA was extracted from 1000-years old cattle bone collected from Ferento, an archaeological site in central Italy. Amplification of 15 microsatellite FAO-recommended markers with PCR products yielded genotypes for four markers. Expected heterozygosity was comparable with values of modern breeds, but observed heterozygosity was underestimated due to allelic loss. Genetic distances suggested a position intermediate between (1) Anatolian, Balkan, Sicilian and South-Italian cattle and (2) the Iberian, North-European and Central-European cattle, but also a clear relationship with two central-Italian breeds, Chianina and Romagnola. This suggests that these breeds are derived from medieval cattle living in the same area. Our results illustrate the potential of ancient DNA for reconstructing the history of local cattle husbandry.

KW - Ancient DNA

KW - Cattle

KW - Ferento

KW - Microsatellite

KW - Neighbornet

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DO - 10.3389/fgene.2015.00068

M3 - Article

AN - SCOPUS:84923667239

VL - 5

JO - Frontiers in genetics

JF - Frontiers in genetics

SN - 1664-8021

IS - FEB

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ER -

Microsatellite genotyping of medieval cattle from central Italy suggests an old origin of Chianina and Romagnola cattle

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Keywords

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