Microtubule-independent movement of the fission yeast nucleus

Research output: Contribution to journalArticlepeer-review

Abstract

Movement of the cell nucleus typically involves the cytoskeleton and either polymerization based pushing forces or motor-based pulling forces. In fission yeast Schizosaccharomyces pombe, nuclear movement and positioning are thought to depend on microtubule polymerization-based pushing forces. Here we describe a novel, microtubule-independent, form of nuclear movement in fission yeast. Microtubule-independent nuclear movement is directed towards growing cell tips, and it is strongest when the nucleus is close to a growing cell tip, and weakest when the nucleus is far from that tip. Microtubule-independent nuclear movement requires actin cables but does not depend on actin polymerization-based pushing or myosin V-based pulling forces. Vesicle-associated membrane protein (VAMP)-associated proteins (VAPs) Scs2 and Scs22, which are critical for endoplasmic reticulum-plasma membrane contact sites in fission yeast, are also required for microtubule-independent nuclear movement. We also find that in cells in which microtubule-based pushing forces are present, disruption of actin cables leads to increased fluctuations in interphase nuclear positioning and subsequent altered septation. Our results suggest two non-exclusive mechanisms for microtubule-independent nuclear movement, which may help illuminate aspects of nuclear positioning in other cells.
Original languageEnglish
Article numberjcs.253021
Number of pages15
JournalJournal of Cell Science
Volume134
Issue number6
Early online date26 Mar 2021
DOIs
Publication statusE-pub ahead of print - 26 Mar 2021

Keywords

  • fission yeast
  • schizsosaccharomyces pombe
  • nucleus
  • nuclear movement
  • actin
  • endoplasmic reticulum

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