miR-29b inhibits TGF-β1-induced cell proliferation in articular chondrocytes

Masahiro Horita, Shun-Neng Hsu, Anna Raper, Colin Farquharson, Louise Stephen

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Transforming growth factor β1 (TGF-β1) is a known regulator of chondrocyte proliferation and promotes cartilage repair in osteoarthritis (OA). microRNA-29b-3p (miR-29b-3p) is downregulated by TGF-β1 and overexpressed in OA cartilage. However, the ability of miR-29b-3p to mediate the chondrocyte pro-proliferative effects of TGF-β1 is not yet understood. This current study aimed to investigate the effect of miR-29b-3p on TGF-β1-induced cell proliferation in murine articular chondrocytes. The stimulation of chondrocytes by TGF-β1 for 24 h resulted in the downregulation of miR-29b-3p expression. The ratio of G0/G1 phase cells decreased in response to TGF-β1 whereas the ratio of S phase cells was increased. Consistent with this observation, miR-29b-3p overexpression inhibited TGF-β1’s ability to promote the ratio of S phase cells and downregulate the ratio of G0/G1 phase cells. These findings suggest that the downregulation of miR-29b-3p is a likely requirement for TGF-β1-mediated proliferation of murine articular chondrocytes. Furthermore, implying that miR-29b-3p expression may be involved in reduced chondrocyte proliferation in OA.
Original languageEnglish
Article number101216
JournalBiochemistry and biophysics reports
Early online date24 Jan 2022
Publication statusPublished - Mar 2022

Keywords / Materials (for Non-textual outputs)

  • Cell proliferation
  • Chondrocyte
  • Osteoarthritis
  • TGF-beta 1
  • microRNA-29b-3p


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