MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity

Arne Peirsman, Eva Blondeel, Tasdiq Ahmed, Jasper Anckaert, Dominique Audenaert, Tom Boterberg, Krisztina Buzas, Neil Carragher, Gastone Castellani, Flávia Castro, Virginie Dangles-marie, John Dawson, Pascal De Tullio, Elly De Vlieghere, Sándor Dedeyne, Herman Depypere, Akos Diosdi, Ruslan I. Dmitriev, Helmut Dolznig, Suzanne FischerChristian Gespach, Vera Goossens, Jyrki Heino, An Hendrix, Peter Horvath, Leoni A. Kunz-schughart, Sebastiaan Maes, Christophe Mangodt, Pieter Mestdagh, Soňa Michlíková, Maria José Oliveira, Francesco Pampaloni, Filippo Piccinini, Cláudio Pinheiro, Jennifer Rahn, Stephen M. Robbins, Elina Siljamäki, Patrick Steigemann, Gwen Sys, Shuichi Takayama, Anna Tesei, Joeri Tulkens, Michiel Van Waeyenberge, Jo Vandesompele, Glenn Wagemans, Claudia Weindorfer, Nurten Yigit, Nina Zablowsky, Michele Zanoni, Phillip Blondeel, Olivier De Wever

Research output: Contribution to journalArticlepeer-review


Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation of this knowledgebase identified heterogeneity in the methodological setup of spheroids. Empirical evaluation and interlaboratory validation of selected variations in spheroid methodology revealed diverse impacts on spheroid metrics. To facilitate interpretation, stimulate transparency and increase awareness, the Consortium defines the MISpheroID string, a minimum set of experimental parameters required to report spheroid research. Thus, MISpheroID combines a valuable resource and a tool for three-dimensional cellular models to mine experimental parameters and to improve reproducibility.
Original languageEnglish
Pages (from-to)1294-1303
JournalNature Methods
Issue number11
Early online date1 Nov 2021
Publication statusE-pub ahead of print - 1 Nov 2021


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