Experimentally observed oscillations in substrate levels of a bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from rat liver were simulated with a mathematical model proposed by the authors earlier and predicting this phenomenon. A nice fit between theory and experiment was obtained for the period, amplitude, and fading of oscillations. Analysis of the model provided a better understanding of the role of separate enzymatic stages in generating the oscillations and regulating the metabolic fluxes in liver. Explanation was given to the critical switchover of glycolytic fluxes upon changes of the influx to the modeled system.
|Number of pages||5|
|Publication status||Published - 1996|