Modification of the classical Lancefield assay of group A streptococcal killing to reduce inter-donor variation

Mark Reglinski; Nicola N Lynskey; Shiranee Sriskandan

doi:10.1016/j.mimet.2016.03.015

Modification of the classical Lancefield assay of group A streptococcal killing to reduce inter-donor variation

Mark Reglinski, Nicola N Lynskey, Shiranee Sriskandan

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

The lack of a surrogate-of-immunity assay presents a major barrier to Streptococcus pyogenes research. Modification of the Lancefield assay to include an antibody digestion step reduced inter-donor variation and permitted detection of the anti-streptococcal activity of intravenous immunoglobulin and convalescent serum, thus facilitating retrospective evaluation of immunity using stored samples.

Original language	English
Pages (from-to)	69-71
Number of pages	3
Journal	Journal of microbiological methods
Volume	124
DOIs	https://doi.org/10.1016/j.mimet.2016.03.015
Publication status	Published - 24 May 2016

Keywords

Antibodies, Bacterial/blood
Bacterial Proteins/immunology
Humans
Immunoassay/methods
Streptococcal Infections/blood
Streptococcus pyogenes/enzymology

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10.1016/j.mimet.2016.03.015

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title = "Modification of the classical Lancefield assay of group A streptococcal killing to reduce inter-donor variation",

abstract = "The lack of a surrogate-of-immunity assay presents a major barrier to Streptococcus pyogenes research. Modification of the Lancefield assay to include an antibody digestion step reduced inter-donor variation and permitted detection of the anti-streptococcal activity of intravenous immunoglobulin and convalescent serum, thus facilitating retrospective evaluation of immunity using stored samples. ",

keywords = "Antibodies, Bacterial/blood, Bacterial Proteins/immunology, Humans, Immunoassay/methods, Streptococcal Infections/blood, Streptococcus pyogenes/enzymology",

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AU - Sriskandan, Shiranee

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KW - Antibodies, Bacterial/blood

KW - Bacterial Proteins/immunology

KW - Humans

KW - Immunoassay/methods

KW - Streptococcal Infections/blood

KW - Streptococcus pyogenes/enzymology

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