Modulation of Strain-Specific Differences in Gene Expression by Cannabinoid Type 2 Receptor Deficiency

Antonia Sophocleous, Andrew H Sims, Aymen I Idris, Stuart H Ralston

Research output: Contribution to journalArticlepeer-review

Abstract

Previous studies have shown that the skeletal consequences of cannabinoid receptor deficiency differ in different strains of mice. In order to explore the mechanisms responsible, we analysed global gene expression in bone from wild-type CD1 mice and littermates with targeted inactivation of the type 2 cannabinoid receptor (Cnr2 (-/-)) and compared the results with those obtained from a similar analysis of wild-type and Cnr2 (-/-) C57BL/6 mice. Trabecular bone volume was increased in Cnr2 (-/-) CD1 mice compared with wild-type littermates but decreased in Cnr2 (-/-) C57BL/6 mice. Microarray analysis identified 354 genes in which substantial differences in gene expression (>1.5-fold) were observed that were specifically affected by Cnr2 deficiency. Bioinformatic analysis of data from wild-type mice of each strain revealed Cnr2-dependent differences in expression of genes clustering within the gene ontology (GO) terms immune response (p 
Original languageEnglish
Pages (from-to)423-432
Number of pages10
JournalCalcified Tissue International
Volume94
Issue number4
DOIs
Publication statusPublished - Apr 2014

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