Projects per year
Abstract / Description of output
Mitosis is a highly regulated process that allows the equal distribution of the genetic material to the daughter cells. Chromosome segregation requires the formation of a bipolar mitotic spindle and assembly of a multi-protein structure termed the kinetochore to mediate attachments between condensed chromosomes and spindle microtubules. In budding yeast, a single microtubule attaches to each kinetochore, necessitating robustness and processivity of this kinetochore-microtubule attachment. The yeast kinetochore localized Dam1 complex forms a direct interaction with the spindle microtubule. In vitro, the Dam1 complex assembles as a ring around microtubules and couples microtubule depolymerization with cargo movement. However, the subunit organization within the Dam1 complex, its higher order oligomerization, and how it interacts with microtubules remain under debate. Here, we used chemical crosslinking and mass spectrometry to define the architecture and subunit organization of the Dam1 complex. This work reveals that both the C termini of Duo1 and Dam1 subunits interact with the microtubule and are critical for microtubule binding of the Dam1 complex, placing Duo1 and Dam1 on the inside of the ring structure. Integrating this information with available structural data, we provide a coherent model for how the Dam1 complex self-assembles around microtubules.
Original language | English |
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Article number | 150237 |
Journal | Open Biology |
Volume | 6 |
DOIs | |
Publication status | Published - 9 Mar 2016 |
Keywords / Materials (for Non-textual outputs)
- Mitosis
- Dam1 complex
- 3D MS crosslinking
- Kinetochore
- Microtubule
- Self-Assembly
Fingerprint
Dive into the research topics of 'Molecular architecture of the Dam1 complex-microtubule 1 interaction'. Together they form a unique fingerprint.Projects
- 4 Finished
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Protein structures in the context of time and space by mass spectrometry
1/06/14 → 31/05/21
Project: Research
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Function and regulatory mechanisms of kinesin holo-complexes in mitosis
1/05/12 → 31/07/18
Project: Research
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Profiles
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Julie Welburn
- School of Biological Sciences - Personal Chair of Mechanistic Cell Biology
- Centre for Engineering Biology
Person: Academic: Research Active