Molecular characterization and immunological roles of avian IL-22 and its soluble receptor IL-22 binding protein

Sungwon Kim, Laura Faris, Chasity M Cox, Lindsay H Sumners, Mark C Jenkins, Ray H Fetterer, Kate B Miska, Rami A Dalloul

Research output: Contribution to journalArticlepeer-review


As a member of the interleukin (IL)-10 family, IL-22 is an important mediator in modulating tissue responses during inflammation. Through activation of STAT3-signaling cascades, IL-22 induces proliferative and anti-apoptotic pathways, as well as antimicrobial peptides (AMPs), that help prevent tissue damage and aid in its repair. This study reports the cloning and expression of recombinant chicken IL-22 (rChIL-22) and its soluble receptor, rChIL22BP, and characterization of biological effects of rChIL-22 during inflammatory responses. Similar to observations with mammalian IL-22, purified rChIL-22 had no effect on either peripheral blood mononuclear cells (PBMCs) or lymphocytes. This was due to the low expression of the receptor ChIL22RA1 chain compared to ChIL10RB chain. rChIL-22 alone did not affect chicken embryo kidney cells (CEKCs); however, co-stimulation of CEKCs with LPS and rChIL-22 enhanced the production of pro-inflammatory cytokines, chemokines and AMPs. Furthermore, rChIL-22 alone stimulated and induced acute phase reactants in chicken embryo liver cells (CELCs). These effects of rChIL-22 were abolished by pre-incubation of rChIL-22 with rChIL22BP. Together, this study indicates an important role of ChIL-22 on epithelial cells and hepatocytes during inflammation.

Original languageEnglish
Pages (from-to)815-27
Number of pages13
Issue number3
Publication statusPublished - Dec 2012


  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cell Proliferation
  • Chick Embryo
  • Chickens
  • Epithelial Cells
  • Fibroblasts
  • Hepatocytes
  • Inflammation
  • Interleukins
  • Leukocytes, Mononuclear
  • Molecular Sequence Data
  • Receptors, Interleukin
  • Recombinant Proteins
  • Sequence Analysis, DNA
  • Tissue Distribution


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