Molecular detection of a putatively novel cyprinid herpesvirus in sichel (Pelecus cultratus) during a mass mortality event in Hungary

Andor Doszpoly; Melitta Papp; Petra P Deákné; Róbert Glávits; Krisztina Ursu; Ádám Dán

doi:10.1007/s00705-015-2348-8

Molecular detection of a putatively novel cyprinid herpesvirus in sichel (Pelecus cultratus) during a mass mortality event in Hungary

Andor Doszpoly, Melitta Papp, Petra P Deákné, Róbert Glávits, Krisztina Ursu, Ádám Dán

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

In the early summer of 2014, mass mortality of sichel (Pelecus cultratus) was observed in Lake Balaton, Hungary. Histological examination revealed degenerative changes within the tubular epithelium, mainly in the distal tubules and collecting ducts in the kidneys and multifocal vacuolisation in the brain stem and cerebellum. Routine molecular investigations showed the presence of the DNA of an unknown alloherpesvirus in some specimens. Subsequently, three genes of the putative herpesviral genome (DNA polymerase, terminase, and helicase) were amplified and partially sequenced. A phylogenetic tree reconstruction based on the concatenated sequence of these three conserved genes implied that the virus belongs to the genus Cyprinivirus within the family Alloherpesviridae. The sequences of the sichel herpesvirus differ markedly from those of the cypriniviruses CyHV-1, CyHV-2 and CyHV-3, putatively representing a fifth species in the genus.

Original language	English
Pages (from-to)	1279-83
Number of pages	5
Journal	Archives of Virology
Volume	160
Issue number	5
DOIs	https://doi.org/10.1007/s00705-015-2348-8
Publication status	Published - May 2015

Keywords / Materials (for Non-textual outputs)

Animal Structures
Animals
Cluster Analysis
Cyprinidae
DNA, Viral
Fish Diseases
Herpesviridae
Herpesviridae Infections
Histocytochemistry
Hungary
Microscopy
Molecular Sequence Data
Phylogeny
Sequence Analysis, DNA
Sequence Homology
Viral Proteins

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10.1007/s00705-015-2348-8

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@article{29289f0b35a74d6192d58e93d702c390,

title = "Molecular detection of a putatively novel cyprinid herpesvirus in sichel (Pelecus cultratus) during a mass mortality event in Hungary",

abstract = "In the early summer of 2014, mass mortality of sichel (Pelecus cultratus) was observed in Lake Balaton, Hungary. Histological examination revealed degenerative changes within the tubular epithelium, mainly in the distal tubules and collecting ducts in the kidneys and multifocal vacuolisation in the brain stem and cerebellum. Routine molecular investigations showed the presence of the DNA of an unknown alloherpesvirus in some specimens. Subsequently, three genes of the putative herpesviral genome (DNA polymerase, terminase, and helicase) were amplified and partially sequenced. A phylogenetic tree reconstruction based on the concatenated sequence of these three conserved genes implied that the virus belongs to the genus Cyprinivirus within the family Alloherpesviridae. The sequences of the sichel herpesvirus differ markedly from those of the cypriniviruses CyHV-1, CyHV-2 and CyHV-3, putatively representing a fifth species in the genus.",

keywords = "Animal Structures, Animals, Cluster Analysis, Cyprinidae, DNA, Viral, Fish Diseases, Herpesviridae, Herpesviridae Infections, Histocytochemistry, Hungary, Microscopy, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Sequence Homology, Viral Proteins",

author = "Andor Doszpoly and Melitta Papp and De{\'a}kn{\'e}, {Petra P} and R{\'o}bert Gl{\'a}vits and Krisztina Ursu and {\'A}d{\'a}m D{\'a}n",

year = "2015",

month = may,

doi = "10.1007/s00705-015-2348-8",

language = "English",

volume = "160",

pages = "1279--83",

journal = "Archives of Virology",

issn = "0304-8608",

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number = "5",

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TY - JOUR

T1 - Molecular detection of a putatively novel cyprinid herpesvirus in sichel (Pelecus cultratus) during a mass mortality event in Hungary

AU - Doszpoly, Andor

AU - Papp, Melitta

AU - Deákné, Petra P

AU - Glávits, Róbert

AU - Ursu, Krisztina

AU - Dán, Ádám

PY - 2015/5

Y1 - 2015/5

N2 - In the early summer of 2014, mass mortality of sichel (Pelecus cultratus) was observed in Lake Balaton, Hungary. Histological examination revealed degenerative changes within the tubular epithelium, mainly in the distal tubules and collecting ducts in the kidneys and multifocal vacuolisation in the brain stem and cerebellum. Routine molecular investigations showed the presence of the DNA of an unknown alloherpesvirus in some specimens. Subsequently, three genes of the putative herpesviral genome (DNA polymerase, terminase, and helicase) were amplified and partially sequenced. A phylogenetic tree reconstruction based on the concatenated sequence of these three conserved genes implied that the virus belongs to the genus Cyprinivirus within the family Alloherpesviridae. The sequences of the sichel herpesvirus differ markedly from those of the cypriniviruses CyHV-1, CyHV-2 and CyHV-3, putatively representing a fifth species in the genus.

AB - In the early summer of 2014, mass mortality of sichel (Pelecus cultratus) was observed in Lake Balaton, Hungary. Histological examination revealed degenerative changes within the tubular epithelium, mainly in the distal tubules and collecting ducts in the kidneys and multifocal vacuolisation in the brain stem and cerebellum. Routine molecular investigations showed the presence of the DNA of an unknown alloherpesvirus in some specimens. Subsequently, three genes of the putative herpesviral genome (DNA polymerase, terminase, and helicase) were amplified and partially sequenced. A phylogenetic tree reconstruction based on the concatenated sequence of these three conserved genes implied that the virus belongs to the genus Cyprinivirus within the family Alloherpesviridae. The sequences of the sichel herpesvirus differ markedly from those of the cypriniviruses CyHV-1, CyHV-2 and CyHV-3, putatively representing a fifth species in the genus.

KW - Animal Structures

KW - Animals

KW - Cluster Analysis

KW - Cyprinidae

KW - DNA, Viral

KW - Fish Diseases

KW - Herpesviridae

KW - Herpesviridae Infections

KW - Histocytochemistry

KW - Hungary

KW - Microscopy

KW - Molecular Sequence Data

KW - Phylogeny

KW - Sequence Analysis, DNA

KW - Sequence Homology

KW - Viral Proteins

U2 - 10.1007/s00705-015-2348-8

DO - 10.1007/s00705-015-2348-8

M3 - Article

C2 - 25643818

SN - 0304-8608

VL - 160

SP - 1279

EP - 1283

JO - Archives of Virology

JF - Archives of Virology

IS - 5

ER -

Molecular detection of a putatively novel cyprinid herpesvirus in sichel (Pelecus cultratus) during a mass mortality event in Hungary

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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