Molecular immunophenotyping of lungs and spleens in naive and vaccinated chickens early after pulmonary avian influenza A (H9N2) virus infection

Winfried G J Degen, Jacqueline Smith, Bartjan Simmelink, Elizabeth J Glass, Dave W Burt, Virgil E J C Schijns

Research output: Contribution to journalArticlepeer-review

Abstract

In a respiratory-infection-model with the avian influenza A H9N2 virus we studied lung and splenic immune reactions in chickens using a recently developed 5K chicken immuno-microarray. Groups of chickens were either mock-immunized (referred to as non-immune), vaccinated with inactivated viral antigen only (immune) or with viral antigen in a water-in-oil (W/O) immunopotentiator (immune potentiated). Three weeks after vaccination all animals were given a respiratory infection. Immune potentiated birds developed inhibitory antiviral antibodies, showed minimal lung histopathology and no detectable viral sequences, while non-immune animals showed microscopic immunopathology and detectable virus. Immune birds, receiving antigen in saline only, showed minimal microscopic histopathology, and intermediate levels of virus detection. These classical features in the different groups were mirrored by overlapping or specific mRNA gene expression profiles in lungs and spleen using microarray analysis. To our knowledge this is the first study demonstrating pneumonia-associated lung pathology of the low pathogenic avian influenza H9N2 virus. Our data provide insights into the molecular interaction of this virus with its natural host when naive or primed by vaccination.
Original languageEnglish
Pages (from-to)6096-109
Number of pages14
JournalVaccine
Volume24
Issue number35-36
DOIs
Publication statusPublished - 28 Aug 2006

Keywords

  • Animals
  • Antigen Presentation/physiology
  • Antigens, CD/metabolism
  • Apoptosis/physiology
  • B-Lymphocytes/metabolism
  • Chickens
  • Complement System Proteins/metabolism
  • Cytokines/metabolism
  • Immunity, Innate
  • Influenza A Virus, H9N2 Subtype/immunology
  • Influenza Vaccines/therapeutic use
  • Influenza in Birds/immunology
  • Influenza in Birds/pathology
  • Influenza in Birds/prevention & control
  • Interferon Type I/metabolism
  • Lung/immunology
  • Lung/pathology
  • Myeloid Cells/metabolism
  • Spleen/immunology
  • Spleen/pathology
  • Toll-Like Receptors/metabolism
  • Transcription Factors/metabolism
  • Tumor Necrosis Factor Receptor-Associated Peptides and Proteins/metabolism
  • Up-Regulation

Fingerprint

Dive into the research topics of 'Molecular immunophenotyping of lungs and spleens in naive and vaccinated chickens early after pulmonary avian influenza A (H9N2) virus infection'. Together they form a unique fingerprint.

Cite this