Abstract
We report the development of a fast and reliable PCR-based method for sex identification of tiger DNA designed to be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently-labelled forward primer and an unlabelled reverse primer, is used to co-amplify homologous fragments of a zinc finger (ZF) protein intron which exhibits size polymorphism between the X and Y chromosomes. The ZFX and ZFY amplicons differ in size by 12 bp and can thus be differentiated by capillary electrophoresis.
Original language | English |
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Pages (from-to) | 299-301 |
Number of pages | 3 |
Journal | Conservation Genetics Resources |
Volume | 4 |
Issue number | 2 |
DOIs | |
Publication status | Published - Jun 2012 |
Externally published | Yes |
Keywords / Materials (for Non-textual outputs)
- Molecular sex identification
- Tiger
- Zinc finger protein
- Forensics
- Felids