Molecular sexing of tigers, Panthera tigris

R. McEwing; K. Ouitavon; J. J. Rovie-Ryan; null Wulansari; F. T. Sitam; R. Ogden

doi:10.1007/s12686-011-9529-x

Molecular sexing of tigers, Panthera tigris

R. McEwing^*, K. Ouitavon, J. J. Rovie-Ryan, Wulansari, F. T. Sitam, R. Ogden

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

We report the development of a fast and reliable PCR-based method for sex identification of tiger DNA designed to be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently-labelled forward primer and an unlabelled reverse primer, is used to co-amplify homologous fragments of a zinc finger (ZF) protein intron which exhibits size polymorphism between the X and Y chromosomes. The ZFX and ZFY amplicons differ in size by 12 bp and can thus be differentiated by capillary electrophoresis.

Original language	English
Pages (from-to)	299-301
Number of pages	3
Journal	Conservation Genetics Resources
Volume	4
Issue number	2
DOIs	https://doi.org/10.1007/s12686-011-9529-x
Publication status	Published - Jun 2012
Externally published	Yes

Keywords

Molecular sex identification
Tiger
Zinc finger protein
Forensics
Felids

Access to Document

10.1007/s12686-011-9529-x

Cite this

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title = "Molecular sexing of tigers, Panthera tigris",

abstract = "We report the development of a fast and reliable PCR-based method for sex identification of tiger DNA designed to be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently-labelled forward primer and an unlabelled reverse primer, is used to co-amplify homologous fragments of a zinc finger (ZF) protein intron which exhibits size polymorphism between the X and Y chromosomes. The ZFX and ZFY amplicons differ in size by 12 bp and can thus be differentiated by capillary electrophoresis.",

keywords = "Molecular sex identification, Tiger, Zinc finger protein, Forensics, Felids",

author = "R. McEwing and K. Ouitavon and Rovie-Ryan, {J. J.} and Wulansari and Sitam, {F. T.} and R. Ogden",

year = "2012",

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doi = "10.1007/s12686-011-9529-x",

language = "English",

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journal = "Conservation Genetics Resources",

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T1 - Molecular sexing of tigers, Panthera tigris

AU - McEwing, R.

AU - Ouitavon, K.

AU - Rovie-Ryan, J. J.

AU - Wulansari, null

AU - Sitam, F. T.

AU - Ogden, R.

PY - 2012/6

Y1 - 2012/6

N2 - We report the development of a fast and reliable PCR-based method for sex identification of tiger DNA designed to be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently-labelled forward primer and an unlabelled reverse primer, is used to co-amplify homologous fragments of a zinc finger (ZF) protein intron which exhibits size polymorphism between the X and Y chromosomes. The ZFX and ZFY amplicons differ in size by 12 bp and can thus be differentiated by capillary electrophoresis.

AB - We report the development of a fast and reliable PCR-based method for sex identification of tiger DNA designed to be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently-labelled forward primer and an unlabelled reverse primer, is used to co-amplify homologous fragments of a zinc finger (ZF) protein intron which exhibits size polymorphism between the X and Y chromosomes. The ZFX and ZFY amplicons differ in size by 12 bp and can thus be differentiated by capillary electrophoresis.

KW - Molecular sex identification

KW - Tiger

KW - Zinc finger protein

KW - Forensics

KW - Felids

U2 - 10.1007/s12686-011-9529-x

DO - 10.1007/s12686-011-9529-x

M3 - Article

VL - 4

SP - 299

EP - 301

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

SN - 1877-7252

IS - 2

ER -