Molecular sexing of tigers, Panthera tigris

R. McEwing*, K. Ouitavon, J. J. Rovie-Ryan, Wulansari, F. T. Sitam, R. Ogden

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

We report the development of a fast and reliable PCR-based method for sex identification of tiger DNA designed to be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently-labelled forward primer and an unlabelled reverse primer, is used to co-amplify homologous fragments of a zinc finger (ZF) protein intron which exhibits size polymorphism between the X and Y chromosomes. The ZFX and ZFY amplicons differ in size by 12 bp and can thus be differentiated by capillary electrophoresis.

Original languageEnglish
Pages (from-to)299-301
Number of pages3
JournalConservation Genetics Resources
Volume4
Issue number2
DOIs
Publication statusPublished - Jun 2012
Externally publishedYes

Keywords

  • Molecular sex identification
  • Tiger
  • Zinc finger protein
  • Forensics
  • Felids

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