Monitoring protein aggregation and toxicity in Alzheimer's disease mouse models using in vivo imaging

Tara L Spires-Jones, Alix de Calignon, Melanie Meyer-Luehmann, Brian J Bacskai, Bradley T Hyman

Research output: Contribution to journalArticlepeer-review

Abstract

Aggregation of amyloid beta peptide into senile plaques and hyperphosphorylated tau protein into neurofibrillary tangles in the brain are the pathological hallmarks of Alzheimer's disease. Despite over a century of research into these lesions, the exact relationship between pathology and neurotoxicity has yet to be fully elucidated. In order to study the formation of plaques and tangles and their effects on the brain, we have applied multiphoton in vivo imaging of transgenic mouse models of Alzheimer's disease. This technique allows longitudinal imaging of pathological aggregation of proteins and the subsequent changes in surrounding neuropil neurodegeneration and recovery after therapeutic interventions.
Original languageEnglish
Pages (from-to)201-7
Number of pages7
JournalMethods
Volume53
Issue number3
DOIs
Publication statusPublished - Mar 2011

Keywords

  • Alzheimer Disease
  • Amyloid
  • Anesthesia
  • Animals
  • Brain
  • Craniotomy
  • Disease Models, Animal
  • Genetic Vectors
  • Humans
  • Image Processing, Computer-Assisted
  • Mice
  • Microscopy, Fluorescence, Multiphoton
  • Neurofibrillary Tangles
  • Plaque, Amyloid

Fingerprint Dive into the research topics of 'Monitoring protein aggregation and toxicity in Alzheimer's disease mouse models using in vivo imaging'. Together they form a unique fingerprint.

Cite this