Monitoring Sinew Contraction During Formation of Tissue-Engineered Fibrin-Based Ligament Constructs

Jennifer Z. Paxton*, Uchena N. G. Wudebwe, Anqi Wang, Daniel Woods, Liam M. Grover

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The ability to study the gross morphological changes occurring during tissue formation is vital to producing tissue-engineered structures of clinically relevant dimensions in vitro. Here, we have used nondestructive methods of digital imaging and optical coherence tomography to monitor the early-stage formation and subsequent maturation of fibrin-based tissue-engineered ligament constructs. In addition, the effect of supplementation with essential promoters of collagen synthesis, ascorbic acid (AA) and proline (P), has been assessed. Contraction of the cell-seeded fibrin gel occurs unevenly within the first 5 days of culture around two fixed anchor points before forming a longitudinal ligament-like construct. AA+P supplementation accelerates gel contraction in the maturation phase of development, producing ligament-like constructs with a higher collagen content and distinct morphology to that of unsupplemented constructs. These studies highlight the importance of being able to control the methods of tissue formation and maturation in vitro to enable the production of tissue-engineered constructs with suitable replacement tissue characteristics for repair of clinical soft-tissue injuries.

Original languageEnglish
Pages (from-to)1596-1607
Number of pages12
JournalTissue Engineering, Part A
Volume18
Issue number15-16
DOIs
Publication statusPublished - Aug 2012

Keywords

  • OPTICAL COHERENCE TOMOGRAPHY
  • DONOR-SITE MORBIDITY
  • L-ASCORBIC-ACID
  • ULTRAHIGH-RESOLUTION
  • GAMMA-RADIATION
  • IN-VITRO
  • TENDON
  • RECONSTRUCTION
  • FIBROBLASTS
  • EXPRESSION

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