Monoclonal antibodies to sheep lymphocytes. I. Identification of MHC class II molecules on lymphoid tissue and changes in the level of class II expression on lymph-borne cells following antigen stimulation in vivo

J Hopkins, B M Dutia, I McConnell

Research output: Contribution to journalArticlepeer-review

Abstract

A rat monoclonal antibody is described that recognizes sheep MHC Class II antigen and appears to recognize a determinant that is non-polymorphic. This antibody precipitates polypeptides of apparent molecular weight 33,000 and 28,000/29,000 (under non-reducing conditions). The binding of this antibody was inhibited by an anti-human DP, DQ, DR beta-chain monoclonal (DA6.231) and its reactivity on murine cells suggests specificity for the I-E gene product. Using this antibody we have studied the distribution of Class II molecules in sheep and also the variations in Class II expression by cells in both peripheral and efferent lymph, produced as a result of in vivo antigen inoculation.
Original languageEnglish
Pages (from-to)433-8
Number of pages6
JournalImmunology
Volume59
Issue number3
Publication statusPublished - Nov 1986

Keywords

  • Animals
  • Antibodies, Monoclonal
  • Antibody Affinity
  • Electrophoresis, Polyacrylamide Gel
  • Epitopes
  • Histocompatibility Antigens Class II
  • Lymphocytes
  • Mitosis
  • Molecular Weight
  • Rats
  • Rats, Inbred Strains
  • Sheep
  • Tissue Distribution

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