Monoclonal antibodies to sheep lymphocytes. I. Identification of MHC class II molecules on lymphoid tissue and changes in the level of class II expression on lymph-borne cells following antigen stimulation in vivo

J Hopkins; B M Dutia; I McConnell

Monoclonal antibodies to sheep lymphocytes. I. Identification of MHC class II molecules on lymphoid tissue and changes in the level of class II expression on lymph-borne cells following antigen stimulation in vivo

J Hopkins, B M Dutia, I McConnell

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

A rat monoclonal antibody is described that recognizes sheep MHC Class II antigen and appears to recognize a determinant that is non-polymorphic. This antibody precipitates polypeptides of apparent molecular weight 33,000 and 28,000/29,000 (under non-reducing conditions). The binding of this antibody was inhibited by an anti-human DP, DQ, DR beta-chain monoclonal (DA6.231) and its reactivity on murine cells suggests specificity for the I-E gene product. Using this antibody we have studied the distribution of Class II molecules in sheep and also the variations in Class II expression by cells in both peripheral and efferent lymph, produced as a result of in vivo antigen inoculation.

Original language	English
Pages (from-to)	433-8
Number of pages	6
Journal	Immunology
Volume	59
Issue number	3
Publication status	Published - Nov 1986

Keywords / Materials (for Non-textual outputs)

Animals
Antibodies, Monoclonal
Antibody Affinity
Electrophoresis, Polyacrylamide Gel
Epitopes
Histocompatibility Antigens Class II
Lymphocytes
Mitosis
Molecular Weight
Rats
Rats, Inbred Strains
Sheep
Tissue Distribution

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@article{1d64d89ae21f4912960066d2768c2c5f,

title = "Monoclonal antibodies to sheep lymphocytes. I. Identification of MHC class II molecules on lymphoid tissue and changes in the level of class II expression on lymph-borne cells following antigen stimulation in vivo",

abstract = "A rat monoclonal antibody is described that recognizes sheep MHC Class II antigen and appears to recognize a determinant that is non-polymorphic. This antibody precipitates polypeptides of apparent molecular weight 33,000 and 28,000/29,000 (under non-reducing conditions). The binding of this antibody was inhibited by an anti-human DP, DQ, DR beta-chain monoclonal (DA6.231) and its reactivity on murine cells suggests specificity for the I-E gene product. Using this antibody we have studied the distribution of Class II molecules in sheep and also the variations in Class II expression by cells in both peripheral and efferent lymph, produced as a result of in vivo antigen inoculation.",

keywords = "Animals, Antibodies, Monoclonal, Antibody Affinity, Electrophoresis, Polyacrylamide Gel, Epitopes, Histocompatibility Antigens Class II, Lymphocytes, Mitosis, Molecular Weight, Rats, Rats, Inbred Strains, Sheep, Tissue Distribution",

author = "J Hopkins and Dutia, {B M} and I McConnell",

year = "1986",

month = nov,

language = "English",

volume = "59",

pages = "433--8",

journal = "Immunology ",

issn = "0019-2805",

publisher = "Wiley-Blackwell",

number = "3",

}

Monoclonal antibodies to sheep lymphocytes. I. Identification of MHC class II molecules on lymphoid tissue and changes in the level of class II expression on lymph-borne cells following antigen stimulation in vivo. / Hopkins, J; Dutia, B M; McConnell, I.
In: Immunology , Vol. 59, No. 3, 11.1986, p. 433-8.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Monoclonal antibodies to sheep lymphocytes. I. Identification of MHC class II molecules on lymphoid tissue and changes in the level of class II expression on lymph-borne cells following antigen stimulation in vivo

AU - Hopkins, J

AU - Dutia, B M

AU - McConnell, I

PY - 1986/11

Y1 - 1986/11

N2 - A rat monoclonal antibody is described that recognizes sheep MHC Class II antigen and appears to recognize a determinant that is non-polymorphic. This antibody precipitates polypeptides of apparent molecular weight 33,000 and 28,000/29,000 (under non-reducing conditions). The binding of this antibody was inhibited by an anti-human DP, DQ, DR beta-chain monoclonal (DA6.231) and its reactivity on murine cells suggests specificity for the I-E gene product. Using this antibody we have studied the distribution of Class II molecules in sheep and also the variations in Class II expression by cells in both peripheral and efferent lymph, produced as a result of in vivo antigen inoculation.

AB - A rat monoclonal antibody is described that recognizes sheep MHC Class II antigen and appears to recognize a determinant that is non-polymorphic. This antibody precipitates polypeptides of apparent molecular weight 33,000 and 28,000/29,000 (under non-reducing conditions). The binding of this antibody was inhibited by an anti-human DP, DQ, DR beta-chain monoclonal (DA6.231) and its reactivity on murine cells suggests specificity for the I-E gene product. Using this antibody we have studied the distribution of Class II molecules in sheep and also the variations in Class II expression by cells in both peripheral and efferent lymph, produced as a result of in vivo antigen inoculation.

KW - Animals

KW - Antibodies, Monoclonal

KW - Antibody Affinity

KW - Electrophoresis, Polyacrylamide Gel

KW - Epitopes

KW - Histocompatibility Antigens Class II

KW - Lymphocytes

KW - Mitosis

KW - Molecular Weight

KW - Rats

KW - Rats, Inbred Strains

KW - Sheep

KW - Tissue Distribution

M3 - Article

C2 - 2431999

SN - 0019-2805

VL - 59

SP - 433

EP - 438

JO - Immunology

JF - Immunology

IS - 3

ER -