Abstract
A monoclonal antibody to the glycolytic enzyme aldolase C was used in an immunoperoxidase technique on unfixed and formalin-fixed paraffin-embedded tissues from the human central nervous system (CNS) and other tissues. No staining outside the CNS was detected. In the central nervous system the antibody gave a selective and intense staining of Purkinje cell perikarya, axons and dendrites in both fixed and unfixed tissues. In the human fetal brain, positive staining of Purkinje cells was seen from 35-36 weeks' gestation onwards. This coincides with the conversion of Purkinje cells to a tetraploid state, and may reflect increased glycolytic activity accompanying the rapid dendritic growth at this stage of development. Preliminary investigations on a variety of disease states suggest that this antibody may be useful in studies of ischaemic damage, congenital abnormalities and degenerative conditions affecting the cerebellum.
Original language | English |
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Pages (from-to) | 11-21 |
Number of pages | 11 |
Journal | Neuropathology and Applied Neurobiology |
Volume | 13 |
Issue number | 1 |
Publication status | Published - 1 Jan 1987 |
Keywords / Materials (for Non-textual outputs)
- Adult
- Antibodies, Monoclonal
- Cerebellum
- Fetus
- Fructose-Bisphosphate Aldolase
- Gestational Age
- Histocytochemistry
- Humans
- Immunoenzyme Techniques
- Purkinje Cells