Morpholino Oligonucleotide Cross-Linked Hydrogels as Portable Optical Oligonucleotide Biosensors

Geraint Langford; Jaclyn Raeburn; David Ferrier; Philip Hands; Michael Shaver

doi:10.1021/acssensors.8b01208

Morpholino Oligonucleotide Cross-Linked Hydrogels as Portable Optical Oligonucleotide Biosensors

Geraint Langford, Jaclyn Raeburn, David Ferrier, Philip Hands, Michael Shaver

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Morpholino Oligonucleotides (MOs), an uncharged DNA analogue, are functionalized with an acrylamide moiety and incorporated into polymer hydrogels as responsive crosslinks for microRNA sequence detection. The MO crosslinks can be selectively cleaved by a short target analyte single-stranded DNA (ssDNA) sequence based on microRNA, inducing a distinct swelling response measured optically. The MO crosslinks offer significant improvement over DNA based systems through improved thermal stability, no salt requirement and 1000-fold improved sensitivity over a comparative biosensor, facilitating a wider range of sensing conditions. Analysis was also achieved using a mobile phone camera, demonstrating portability.

Original language	English
Pages (from-to)	185-191
Number of pages	7
Journal	ACS Sensors
Volume	4
Issue number	1
Early online date	28 Dec 2018
DOIs	https://doi.org/10.1021/acssensors.8b01208
Publication status	Published - 25 Jan 2019

Access to Document

10.1021/acssensors.8b01208

MOCHS - ACS - R v4.1Accepted author manuscript, 741 KB

https://pubs.acs.org/doi/10.1021/acssensors.8b01208

5 Finished

Swellable Hydrogels as miRNA Sensors
Shaver, M.
UK industry, commerce and public corporations
1/09/15 → 28/02/19
Project: Research
Point-of-care miRNA sensors for disease diagnosticsNew Costing for Barry Turner - Linked to Chemistry 035508
Hands, P. & Shaver, M.
UK industry, commerce and public corporations
1/07/15 → 30/06/16
Project: Research
MRC Confidence in Concept
Hands, P.
MRC
1/07/15 → 30/06/16
Project: Research

1 Article

Conductive composites for oligonucleotide detection
Ferrier, D., Shaver, M. & Hands, P., 1 Feb 2018, In: Sensing and Bio-Sensing Research. 17, p. 1-6
Research output: Contribution to journal › Article › peer-review

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title = "Morpholino Oligonucleotide Cross-Linked Hydrogels as Portable Optical Oligonucleotide Biosensors",

abstract = "Morpholino Oligonucleotides (MOs), an uncharged DNA analogue, are functionalized with an acrylamide moiety and incorporated into polymer hydrogels as responsive crosslinks for microRNA sequence detection. The MO crosslinks can be selectively cleaved by a short target analyte single-stranded DNA (ssDNA) sequence based on microRNA, inducing a distinct swelling response measured optically. The MO crosslinks offer significant improvement over DNA based systems through improved thermal stability, no salt requirement and 1000-fold improved sensitivity over a comparative biosensor, facilitating a wider range of sensing conditions. Analysis was also achieved using a mobile phone camera, demonstrating portability.",

author = "Geraint Langford and Jaclyn Raeburn and David Ferrier and Philip Hands and Michael Shaver",

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AU - Raeburn, Jaclyn

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AU - Hands, Philip

AU - Shaver, Michael

PY - 2019/1/25

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N2 - Morpholino Oligonucleotides (MOs), an uncharged DNA analogue, are functionalized with an acrylamide moiety and incorporated into polymer hydrogels as responsive crosslinks for microRNA sequence detection. The MO crosslinks can be selectively cleaved by a short target analyte single-stranded DNA (ssDNA) sequence based on microRNA, inducing a distinct swelling response measured optically. The MO crosslinks offer significant improvement over DNA based systems through improved thermal stability, no salt requirement and 1000-fold improved sensitivity over a comparative biosensor, facilitating a wider range of sensing conditions. Analysis was also achieved using a mobile phone camera, demonstrating portability.

AB - Morpholino Oligonucleotides (MOs), an uncharged DNA analogue, are functionalized with an acrylamide moiety and incorporated into polymer hydrogels as responsive crosslinks for microRNA sequence detection. The MO crosslinks can be selectively cleaved by a short target analyte single-stranded DNA (ssDNA) sequence based on microRNA, inducing a distinct swelling response measured optically. The MO crosslinks offer significant improvement over DNA based systems through improved thermal stability, no salt requirement and 1000-fold improved sensitivity over a comparative biosensor, facilitating a wider range of sensing conditions. Analysis was also achieved using a mobile phone camera, demonstrating portability.

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Morpholino Oligonucleotide Cross-Linked Hydrogels as Portable Optical Oligonucleotide Biosensors

Abstract / Description of output

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Projects

Swellable Hydrogels as miRNA Sensors

Point-of-care miRNA sensors for disease diagnosticsNew Costing for Barry Turner - Linked to Chemistry 035508

MRC Confidence in Concept

Research output

Conductive composites for oligonucleotide detection

Cite this