Morpholino Oligonucleotide Cross-Linked Hydrogels as Portable Optical Oligonucleotide Biosensors

Geraint Langford, Jaclyn Raeburn, David Ferrier, Philip Hands, Michael Shaver

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Morpholino Oligonucleotides (MOs), an uncharged DNA analogue, are functionalized with an acrylamide moiety and incorporated into polymer hydrogels as responsive crosslinks for microRNA sequence detection. The MO crosslinks can be selectively cleaved by a short target analyte single-stranded DNA (ssDNA) sequence based on microRNA, inducing a distinct swelling response measured optically. The MO crosslinks offer significant improvement over DNA based systems through improved thermal stability, no salt requirement and 1000-fold improved sensitivity over a comparative biosensor, facilitating a wider range of sensing conditions. Analysis was also achieved using a mobile phone camera, demonstrating portability.
Original languageEnglish
Pages (from-to)185-191
Number of pages7
JournalACS Sensors
Issue number1
Early online date28 Dec 2018
Publication statusPublished - 25 Jan 2019


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