Mosaic analysis of stem cell function and wound healing in the mouse corneal epithelium

Richard L Mort; Thaya Ramaesh; Dirk A Kleinjan; Steven D Morley; John D West

doi:10.1186/1471-213X-9-4

Mosaic analysis of stem cell function and wound healing in the mouse corneal epithelium

Richard L Mort, Thaya Ramaesh, Dirk A Kleinjan, Steven D Morley, John D West

Research output: Contribution to journal › Article › peer-review

Abstract

The mouse corneal epithelium is a continuously renewing 5-6 cell thick protective layer covering the corneal surface, which regenerates rapidly when injured. It is maintained by peripherally located limbal stem cells (LSCs) that produce transient amplifying cells (TACs) which proliferate, migrate centripetally, differentiate and are eventually shed from the epithelial surface. LSC activity is required both for normal tissue maintenance and wound healing. Mosaic analysis can provide insights into LSC function, cell movement and cell mixing during tissue maintenance and repair. The present study investigates cell streaming during corneal maintenance and repair and changes in LSC function with age.

Original language	English
Pages (from-to)	4
Journal	BMC Developmental Biology
Volume	9
DOIs	https://doi.org/10.1186/1471-213X-9-4
Publication status	Published - 2009

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10.1186/1471-213X-9-4

Mosaic analysis of stem cell function and wound healing in the mouse corneal epithelium
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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title = "Mosaic analysis of stem cell function and wound healing in the mouse corneal epithelium",

abstract = "The mouse corneal epithelium is a continuously renewing 5-6 cell thick protective layer covering the corneal surface, which regenerates rapidly when injured. It is maintained by peripherally located limbal stem cells (LSCs) that produce transient amplifying cells (TACs) which proliferate, migrate centripetally, differentiate and are eventually shed from the epithelial surface. LSC activity is required both for normal tissue maintenance and wound healing. Mosaic analysis can provide insights into LSC function, cell movement and cell mixing during tissue maintenance and repair. The present study investigates cell streaming during corneal maintenance and repair and changes in LSC function with age.",

author = "Mort, {Richard L} and Thaya Ramaesh and Kleinjan, {Dirk A} and Morley, {Steven D} and West, {John D}",

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AU - Morley, Steven D

AU - West, John D

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Mosaic analysis of stem cell function and wound healing in the mouse corneal epithelium

Abstract

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