Motif-grafted antibodies containing the replicative interface of cellular PrP are specific for PrPSc

G Moroncini, N Kanu, L Solforosi, G Abalos, GC Telling, Mark Head, James Ironside, JP Brockes, DR Burton, RA Williams

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Prion diseases are closely associated with the conversion of the cellular prion protein (PrPC) to an abnormal conformer (PrPSc) [Prusiner, S. B. (1998) Proc. Natl. Acad. Sci. USA 95, 13363-13383]. Monoclonal antibodies that bind epitopes comprising residues 96-104 and 133-158 of PrPC potently inhibit this process, presumably by preventing heterodimeric association of PrPC and PrPSc, and suggest that these regions of PrPC may be critical components of the PrPC-PrPSc replicative interface. We reasoned that transplanting PrP sequence corresponding to these regions into a suitable carrier molecule, such as an antibody, could impart specific recognition of disease-associated forms of PrP. To test this hypothesis, polypeptides containing PrP sequence between residues 89-112 or 136-158 were used to replace the extended heavy chain complementarity-determining region 3 of an IgG antibody specific for the envelope glycoprotein of HIV-1. Herein the resulting engineered PrP-IgGs are shown to bind specifically to infective fractions of PrP in mouse, human, and hamster prion-infected tissues, but not to PrPC, other cellular components, or the HIV-1 envelope. PrPSc reactivity was abolished when the sequence of the PrP 89-112 and 136-158 grafts was mutated, scrambled, or N-terminally truncated. Our findings suggest that residues within the 89-112 and 136-158 segments of PrPC are key components of one face of the PrPC-PrPSc complex. PrPSc-specific antibodies produced by the approach described may find widespread application in the study of prion biology and replication and in the detection of infectious prions in human and animal materials.

Original languageEnglish
Pages (from-to)10404-10409
Number of pages6
JournalProceedings of the National Academy of Sciences (PNAS)
Issue number28
Publication statusPublished - 13 Jul 2004

Keywords / Materials (for Non-textual outputs)

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Antibody Specificity
  • Creutzfeldt-Jakob Syndrome
  • Cricetinae
  • Epitopes
  • Humans
  • Immunoglobulin G
  • Mice
  • Molecular Sequence Data
  • PrPC Proteins
  • PrPSc Proteins
  • Scrapie


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