Mouse embryonic head as a site for hematopoietic stem cell development

Zhuan Li, Yu Lan, Wenyan He, Dongbo Chen, Jun Wang, Fan Zhou, Yu Wang, Huayan Sun, Xianda Chen, Chunhong Xu, Sha Li, Yakun Pang, Guangzhou Zhang, Liping Yang, Lingling Zhu, Ming Fan, Aijia Shang, Zhenyu Ju, Lingfei Luo, Yuqiang DingWei Guo, Weiping Yuan, Xiao Yang, Bing Liu

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

In the mouse embryo, the aorta-gonad-mesonephros (AGM) region is considered to be the sole location for intraembryonic emergence of hematopoietic stem cells (HSCs). Here we report that, in parallel to the AGM region, the E10.5-E11.5 mouse head harbors bona fide HSCs, as defined by long-term, high-level, multilineage reconstitution and self-renewal capacity in adult recipients, before HSCs enter the circulation. The presence of hemogenesis in the midgestation head is indicated by the appearance of intravascular cluster cells and the blood-forming capacity of a sorted endothelial cell population. In addition, lineage tracing via an inducible VE-cadherin-Cre transgene demonstrates the hemogenic capacity of head endothelium. Most importantly, a spatially restricted lineage labeling system reveals the physiological contribution of cerebrovascular endothelium to postnatal HSCs and multilineage hematopoiesis. We conclude that the mouse embryonic head is a previously unappreciated site for HSC emergence within the developing embryo.

Original languageEnglish
Pages (from-to)663-75
Number of pages13
JournalCell Stem Cell
Issue number5
Publication statusPublished - 2 Nov 2012

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Antigens, CD
  • Aorta
  • Cadherins
  • Cell Differentiation
  • Cell Lineage
  • Embryo, Mammalian
  • Endothelium, Vascular
  • Gonads
  • Head
  • Hematopoietic Stem Cells
  • Mesonephros
  • Mice
  • Transgenes


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