Movement-specific signaling is differentially distributed across motor cortex layer 5 projection neuron classes

Stephen Currie; Julian Ammer; Brian Premchand; Joshua Dacre; Yufei Wu; Constantinos Eleftheriou; Matt Colligan; Thomas Clarke; Leah Mitchell; Aldo  Faisal; Matthias H Hennig; Ian Duguid

doi:10.1016/j.celrep.2022.110801

Movement-specific signaling is differentially distributed across motor cortex layer 5 projection neuron classes

Stephen Currie, Julian Ammer, Brian Premchand, Joshua Dacre, Yufei Wu, Constantinos Eleftheriou, Matt Colligan, Thomas Clarke, Leah Mitchell, Aldo Faisal, Matthias H Hennig, Ian Duguid

Research output: Contribution to journal › Article › peer-review

Original language	English
Journal	Cell Reports
Early online date	10 May 2022
DOIs	https://doi.org/10.1016/j.celrep.2022.110801
Publication status	E-pub ahead of print - 10 May 2022

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10.1016/j.celrep.2022.110801

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@article{cfd1658eab23458bb02c71648c1d5e76,

title = "Movement-specific signaling is differentially distributed across motor cortex layer 5 projection neuron classes",

author = "Stephen Currie and Julian Ammer and Brian Premchand and Joshua Dacre and Yufei Wu and Constantinos Eleftheriou and Matt Colligan and Thomas Clarke and Leah Mitchell and Aldo Faisal and Hennig, {Matthias H} and Ian Duguid",

note = "Funding Information: We are grateful to G{\"u}lsen S{\"u}rmeli, Matt Nolan, and members of the Nolan, Duguid, and S{\"u}rmeli labs for experimental discussions and comments on the manuscript; Nick Steinmetz for the suggested design of the author contribution matrix; and Tiago Branco and Kostas Betsios for Mantis64. AAV-GCaMP6s was a gift from Douglas Kim and the GENIE Project (Addgene 100844-AAV1). AAV-pgk-Cre was a gift from Patrick Aebischer (Addgene viral prep 24593-AAVrg). pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA was a gift from Tobias Bonhoeffer, Mark Huebener, and Tobias Rose (Addgene viral prep 68717-AAV1). Confocal microscopy was performed in the IMPACT Imaging Facility at the University of Edinburgh. Research was supported by grants from the Biotechnology and Biological Sciences Research Council ( BB/R018537/1 to I.D.), the DFG fellowship program ( AM 443/1-1 to J.J.A.), the Shirley Foundation , a Simons Initiative for the Developing Brain (SIDB) PhD studentship (to C.E.), an A ∗ STAR PhD studentship (to B.P.), and a Wellcome Senior Research Fellowship ( 110131/Z/15/Z to I.D.). Funding Information: We are grateful to G{\"u}lsen S{\"u}rmeli, Matt Nolan, and members of the Nolan, Duguid, and S{\"u}rmeli labs for experimental discussions and comments on the manuscript; Nick Steinmetz for the suggested design of the author contribution matrix; and Tiago Branco and Kostas Betsios for Mantis64. AAV-GCaMP6s was a gift from Douglas Kim and the GENIE Project (Addgene 100844-AAV1). AAV-pgk-Cre was a gift from Patrick Aebischer (Addgene viral prep 24593-AAVrg). pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA was a gift from Tobias Bonhoeffer, Mark Huebener, and Tobias Rose (Addgene viral prep 68717-AAV1). Confocal microscopy was performed in the IMPACT Imaging Facility at the University of Edinburgh. Research was supported by grants from the Biotechnology and Biological Sciences Research Council (BB/R018537/1 to I.D.), the DFG fellowship program (AM 443/1-1 to J.J.A.), the Shirley Foundation, a Simons Initiative for the Developing Brain (SIDB) PhD studentship (to C.E.), an A∗STAR PhD studentship (to B.P.), and a Wellcome Senior Research Fellowship (110131/Z/15/Z to I.D.). Conceptualization, B.P. J.J.A. and I.D.; methodology & investigation, S.P.C. J.J.A. B.P. J.D. Y.W. C.E. M.C. T.C. L.M. M.H.H. and I.D.; resources, J.J.A. Y.W. M.C. A.A.F. and M.H.H.; review & editing, all authors. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

month = may,

day = "10",

doi = "10.1016/j.celrep.2022.110801",

language = "English",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

}

TY - JOUR

T1 - Movement-specific signaling is differentially distributed across motor cortex layer 5 projection neuron classes

AU - Currie, Stephen

AU - Ammer, Julian

AU - Premchand, Brian

AU - Dacre, Joshua

AU - Wu, Yufei

AU - Eleftheriou, Constantinos

AU - Colligan, Matt

AU - Clarke, Thomas

AU - Mitchell, Leah

AU - Faisal, Aldo

AU - Hennig, Matthias H

AU - Duguid, Ian

N1 - Funding Information: We are grateful to Gülsen Sürmeli, Matt Nolan, and members of the Nolan, Duguid, and Sürmeli labs for experimental discussions and comments on the manuscript; Nick Steinmetz for the suggested design of the author contribution matrix; and Tiago Branco and Kostas Betsios for Mantis64. AAV-GCaMP6s was a gift from Douglas Kim and the GENIE Project (Addgene 100844-AAV1). AAV-pgk-Cre was a gift from Patrick Aebischer (Addgene viral prep 24593-AAVrg). pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA was a gift from Tobias Bonhoeffer, Mark Huebener, and Tobias Rose (Addgene viral prep 68717-AAV1). Confocal microscopy was performed in the IMPACT Imaging Facility at the University of Edinburgh. Research was supported by grants from the Biotechnology and Biological Sciences Research Council ( BB/R018537/1 to I.D.), the DFG fellowship program ( AM 443/1-1 to J.J.A.), the Shirley Foundation , a Simons Initiative for the Developing Brain (SIDB) PhD studentship (to C.E.), an A ∗ STAR PhD studentship (to B.P.), and a Wellcome Senior Research Fellowship ( 110131/Z/15/Z to I.D.). Funding Information: We are grateful to Gülsen Sürmeli, Matt Nolan, and members of the Nolan, Duguid, and Sürmeli labs for experimental discussions and comments on the manuscript; Nick Steinmetz for the suggested design of the author contribution matrix; and Tiago Branco and Kostas Betsios for Mantis64. AAV-GCaMP6s was a gift from Douglas Kim and the GENIE Project (Addgene 100844-AAV1). AAV-pgk-Cre was a gift from Patrick Aebischer (Addgene viral prep 24593-AAVrg). pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA was a gift from Tobias Bonhoeffer, Mark Huebener, and Tobias Rose (Addgene viral prep 68717-AAV1). Confocal microscopy was performed in the IMPACT Imaging Facility at the University of Edinburgh. Research was supported by grants from the Biotechnology and Biological Sciences Research Council (BB/R018537/1 to I.D.), the DFG fellowship program (AM 443/1-1 to J.J.A.), the Shirley Foundation, a Simons Initiative for the Developing Brain (SIDB) PhD studentship (to C.E.), an A∗STAR PhD studentship (to B.P.), and a Wellcome Senior Research Fellowship (110131/Z/15/Z to I.D.). Conceptualization, B.P. J.J.A. and I.D.; methodology & investigation, S.P.C. J.J.A. B.P. J.D. Y.W. C.E. M.C. T.C. L.M. M.H.H. and I.D.; resources, J.J.A. Y.W. M.C. A.A.F. and M.H.H.; review & editing, all authors. The authors declare no competing interests. Publisher Copyright: © 2022 The Authors

PY - 2022/5/10

Y1 - 2022/5/10

U2 - 10.1016/j.celrep.2022.110801

DO - 10.1016/j.celrep.2022.110801

M3 - Article

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

ER -