MSI-low, a real phenomenon which varies in frequency among cancer types

Sarah E R Halford; Elinor J Sawyer; Maryou B Lambros; Patricia Gorman; Nicola D Macdonald; Ian C Talbot; William D Foulkes; Cheryl E Gillett; Diana M Barnes; Lars A Akslen; Kwok Lee; Ian J Jacobs; Andrew M Hanby; Trivadi S Ganesan; Helga B Salvesen; Walter F Bodmer; Ian P M Tomlinson; Rebecca R Roylance

doi:10.1002/path.1453

MSI-low, a real phenomenon which varies in frequency among cancer types

Sarah E R Halford, Elinor J Sawyer, Maryou B Lambros, Patricia Gorman, Nicola D Macdonald, Ian C Talbot, William D Foulkes, Cheryl E Gillett, Diana M Barnes, Lars A Akslen, Kwok Lee, Ian J Jacobs, Andrew M Hanby, Trivadi S Ganesan, Helga B Salvesen, Walter F Bodmer, Ian P M Tomlinson, Rebecca R Roylance

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

This study assessed whether low-level microsatellite instability (MSI-L) is a phenomenon specific to colorectal cancers or is also present in other tumour types. Breast (grade III ductal and lobular), endometrial and ovarian carcinomas, as well as colorectal cancers, were analysed for MSI-L using eight microsatellite markers. The markers were selected from a panel that had previously been shown to be sensitive for the detection of MSI-L in colorectal cancers. It was found that MSI-L was present in 30 of 87 (35%) colorectal cancers, 2 of 59 (3%) grade III breast carcinomas, 1 of 35 (3%) lobular breast cancers, 16 of 50 (32%) endometrial cancers, and 9 of 34 (26%) ovarian cancers. These results suggest that MSI-L is a very rare occurrence in breast carcinomas, but does occur as a real phenomenon in colorectal, endometrial, and ovarian carcinomas, which are all part of the hereditary non-polyposis colon cancer (HNPCC) syndrome. PCR artefact was also found to masquerade as MSI-L; criteria for the assessment of MSI-L are suggested to eliminate this problem.

Original language	English
Pages (from-to)	389-94
Number of pages	6
Journal	The Journal of Pathology
Volume	201
Issue number	3
DOIs	https://doi.org/10.1002/path.1453
Publication status	Published - Nov 2003

Keywords / Materials (for Non-textual outputs)

Biomarkers, Tumor/genetics
Breast Neoplasms/genetics
Colorectal Neoplasms/genetics
Colorectal Neoplasms, Hereditary Nonpolyposis/genetics
Endometrial Neoplasms/genetics
Female
Genetic Markers/genetics
Humans
Microsatellite Repeats/genetics
Neoplasm Staging
Ovarian Neoplasms/genetics
Polymerase Chain Reaction/methods

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10.1002/path.1453

Cite this

Halford, S. E. R., Sawyer, E. J., Lambros, M. B., Gorman, P., Macdonald, N. D., Talbot, I. C., Foulkes, W. D., Gillett, C. E., Barnes, D. M., Akslen, L. A., Lee, K., Jacobs, I. J., Hanby, A. M., Ganesan, T. S., Salvesen, H. B., Bodmer, W. F., Tomlinson, I. P. M., & Roylance, R. R. (2003). MSI-low, a real phenomenon which varies in frequency among cancer types. The Journal of Pathology, 201(3), 389-94. https://doi.org/10.1002/path.1453

@article{ffa04a1a37ba4101863ea76ea9725005,

title = "MSI-low, a real phenomenon which varies in frequency among cancer types",

abstract = "This study assessed whether low-level microsatellite instability (MSI-L) is a phenomenon specific to colorectal cancers or is also present in other tumour types. Breast (grade III ductal and lobular), endometrial and ovarian carcinomas, as well as colorectal cancers, were analysed for MSI-L using eight microsatellite markers. The markers were selected from a panel that had previously been shown to be sensitive for the detection of MSI-L in colorectal cancers. It was found that MSI-L was present in 30 of 87 (35%) colorectal cancers, 2 of 59 (3%) grade III breast carcinomas, 1 of 35 (3%) lobular breast cancers, 16 of 50 (32%) endometrial cancers, and 9 of 34 (26%) ovarian cancers. These results suggest that MSI-L is a very rare occurrence in breast carcinomas, but does occur as a real phenomenon in colorectal, endometrial, and ovarian carcinomas, which are all part of the hereditary non-polyposis colon cancer (HNPCC) syndrome. PCR artefact was also found to masquerade as MSI-L; criteria for the assessment of MSI-L are suggested to eliminate this problem.",

keywords = "Biomarkers, Tumor/genetics, Breast Neoplasms/genetics, Colorectal Neoplasms/genetics, Colorectal Neoplasms, Hereditary Nonpolyposis/genetics, Endometrial Neoplasms/genetics, Female, Genetic Markers/genetics, Humans, Microsatellite Repeats/genetics, Neoplasm Staging, Ovarian Neoplasms/genetics, Polymerase Chain Reaction/methods",

author = "Halford, {Sarah E R} and Sawyer, {Elinor J} and Lambros, {Maryou B} and Patricia Gorman and Macdonald, {Nicola D} and Talbot, {Ian C} and Foulkes, {William D} and Gillett, {Cheryl E} and Barnes, {Diana M} and Akslen, {Lars A} and Kwok Lee and Jacobs, {Ian J} and Hanby, {Andrew M} and Ganesan, {Trivadi S} and Salvesen, {Helga B} and Bodmer, {Walter F} and Tomlinson, {Ian P M} and Roylance, {Rebecca R}",

year = "2003",

month = nov,

doi = "10.1002/path.1453",

language = "English",

volume = "201",

pages = "389--94",

journal = "The Journal of Pathology",

issn = "0022-3417",

publisher = "Wiley",

number = "3",

}

Halford, SER, Sawyer, EJ, Lambros, MB, Gorman, P, Macdonald, ND, Talbot, IC, Foulkes, WD, Gillett, CE, Barnes, DM, Akslen, LA, Lee, K, Jacobs, IJ, Hanby, AM, Ganesan, TS, Salvesen, HB, Bodmer, WF, Tomlinson, IPM & Roylance, RR 2003, 'MSI-low, a real phenomenon which varies in frequency among cancer types', The Journal of Pathology, vol. 201, no. 3, pp. 389-94. https://doi.org/10.1002/path.1453

TY - JOUR

T1 - MSI-low, a real phenomenon which varies in frequency among cancer types

AU - Halford, Sarah E R

AU - Sawyer, Elinor J

AU - Lambros, Maryou B

AU - Gorman, Patricia

AU - Macdonald, Nicola D

AU - Talbot, Ian C

AU - Foulkes, William D

AU - Gillett, Cheryl E

AU - Barnes, Diana M

AU - Akslen, Lars A

AU - Lee, Kwok

AU - Jacobs, Ian J

AU - Hanby, Andrew M

AU - Ganesan, Trivadi S

AU - Salvesen, Helga B

AU - Bodmer, Walter F

AU - Tomlinson, Ian P M

AU - Roylance, Rebecca R

PY - 2003/11

Y1 - 2003/11

N2 - This study assessed whether low-level microsatellite instability (MSI-L) is a phenomenon specific to colorectal cancers or is also present in other tumour types. Breast (grade III ductal and lobular), endometrial and ovarian carcinomas, as well as colorectal cancers, were analysed for MSI-L using eight microsatellite markers. The markers were selected from a panel that had previously been shown to be sensitive for the detection of MSI-L in colorectal cancers. It was found that MSI-L was present in 30 of 87 (35%) colorectal cancers, 2 of 59 (3%) grade III breast carcinomas, 1 of 35 (3%) lobular breast cancers, 16 of 50 (32%) endometrial cancers, and 9 of 34 (26%) ovarian cancers. These results suggest that MSI-L is a very rare occurrence in breast carcinomas, but does occur as a real phenomenon in colorectal, endometrial, and ovarian carcinomas, which are all part of the hereditary non-polyposis colon cancer (HNPCC) syndrome. PCR artefact was also found to masquerade as MSI-L; criteria for the assessment of MSI-L are suggested to eliminate this problem.

AB - This study assessed whether low-level microsatellite instability (MSI-L) is a phenomenon specific to colorectal cancers or is also present in other tumour types. Breast (grade III ductal and lobular), endometrial and ovarian carcinomas, as well as colorectal cancers, were analysed for MSI-L using eight microsatellite markers. The markers were selected from a panel that had previously been shown to be sensitive for the detection of MSI-L in colorectal cancers. It was found that MSI-L was present in 30 of 87 (35%) colorectal cancers, 2 of 59 (3%) grade III breast carcinomas, 1 of 35 (3%) lobular breast cancers, 16 of 50 (32%) endometrial cancers, and 9 of 34 (26%) ovarian cancers. These results suggest that MSI-L is a very rare occurrence in breast carcinomas, but does occur as a real phenomenon in colorectal, endometrial, and ovarian carcinomas, which are all part of the hereditary non-polyposis colon cancer (HNPCC) syndrome. PCR artefact was also found to masquerade as MSI-L; criteria for the assessment of MSI-L are suggested to eliminate this problem.

KW - Biomarkers, Tumor/genetics

KW - Breast Neoplasms/genetics

KW - Colorectal Neoplasms/genetics

KW - Colorectal Neoplasms, Hereditary Nonpolyposis/genetics

KW - Endometrial Neoplasms/genetics

KW - Female

KW - Genetic Markers/genetics

KW - Humans

KW - Microsatellite Repeats/genetics

KW - Neoplasm Staging

KW - Ovarian Neoplasms/genetics

KW - Polymerase Chain Reaction/methods

U2 - 10.1002/path.1453

DO - 10.1002/path.1453

M3 - Article

C2 - 14595750

SN - 0022-3417

VL - 201

SP - 389

EP - 394

JO - The Journal of Pathology

JF - The Journal of Pathology

IS - 3

ER -

MSI-low, a real phenomenon which varies in frequency among cancer types

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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