Projects per year
Abstract / Description of output
Living cells are heterogeneous and rapidly changing biological samples. It is thus desirable to measure molecular concentration and dynamics in many locations at the same time. In this note, we present a multi-confocal setup capable of performing simultaneous fluorescence correlation spectroscopy measurements, by focusing the spots with a spatial light modulator and acquiring data with a monolithic 32 × 32 single-photon avalanche photodiode array. A post-processing method is proposed to correct cross-talk effects between neighboring spots. We demonstrate the applicability of our system by simultaneously measuring the diffusion of free enhanced Green Fluorescent Protein (eGFP) molecules at nine different points in living cells.
Original language | English |
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Pages (from-to) | 076105 - 076105-3 |
Number of pages | 3 |
Journal | Review of Scientific Instruments |
Volume | 84 |
Issue number | 7 |
DOIs | |
Publication status | Published - Jul 2013 |
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Dive into the research topics of 'Multi-confocal fluorescence correlation spectroscopy in living cells using a complementary metal oxide semiconductor-single photon avalanche diode array'. Together they form a unique fingerprint.Projects
- 2 Finished
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MM-FLIM: Multiplexed multiphoton fluorescence lifetime microscopy
Henderson, R. & Ameer-Beg, S.
1/10/11 → 30/09/14
Project: Research
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MEGAFRAME: MEGAFRAME: Million Frame Per Second, Time Correlated Single Photon Camera
Henderson, R. & Crain, J.
1/06/06 → 30/04/10
Project: Research