Multi-species meta-analysis identifies transcriptional signatures associated with cardiac endothelial responses in the ischaemic heart

Cass Li, Emmanouil Solomonidis, Bronwyn Berkeley, Michelle Tang, Katherine Ross Stewart, Daniel Perez-Vicencio, Ian McCracken, Ana-Mishel Spiroski, Gillian A Gray, Anna Barton, Stephanie L Sellers, Paul R Riley, Andrew H Baker, Mairi Brittan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Myocardial infarction remains the leading cause of heart failure. The adult human heart lacks the capacity to undergo endogenous regeneration. New blood vessel growth is integral to regenerative medicine necessitating a comprehensive understanding of the pathways that regulate vascular regeneration. We present a comprehensive meta-analysis of integrated single cell RNA-sequencing data of coronary vascular endothelial cells from the developing and adult mouse and human heart spanning healthy and acute and chronic ischaemic cardiac disease. We identify species-conserved gene regulatory pathways aligned to endogenous neovascularisation. We annotated injury-associated temporal shifts of the endothelial transcriptome and validated four genes: VEGF-C, KLF4, EGR1 and ZFP36. Moreover, we show that ZFP36 regulates human coronary endothelial cell proliferation and define that VEGF-C administration in vivo enhances clonal expansion of the cardiac vasculature post-myocardial infarction. Finally, we constructed a coronary endothelial cell meta-atlas, CrescENDO, to empower future in-depth research to target pathways associated with coronary neovascularisation.
Original languageEnglish
JournalCardiovascular Research
Early online date9 Sep 2022
DOIs
Publication statusE-pub ahead of print - 9 Sep 2022

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