Multiple embryo time-lapse imaging of zebrafish development

Leah Herrgen, Christian Schröter, Lola Bajard, Andrew C Oates

Research output: Contribution to journalArticlepeer-review

Abstract

Understanding the dynamics of developmental and cellular processes requires documentation of their changes with appropriate temporal and spatial resolution. Furthermore, simultaneous recording from a population of embryos under identical conditions allows statistical estimates of precision and variability to be made. This chapter describes a protocol for time-lapse microscopy of multiple embryos in parallel developing under tightly controlled conditions. This method is currently best suited to follow tissue-scale morphogenetic movements with temporal resolution in the minute range, for hours or even days. Applications of the method include the comparison of the dynamics of a process of interest between groups of wild-type embryos and their mutant siblings or between embryos treated with different chemical compounds. Temperature control allows for the investigation of the temperature dependence of a process of interest.

Original languageEnglish
Pages (from-to)243-54
Number of pages12
JournalMethods in Molecular Biology
Volume546
DOIs
Publication statusPublished - 2009

Keywords

  • Animals
  • Developmental Biology
  • Embryo, Nonmammalian
  • Embryonic Development
  • Image Processing, Computer-Assisted
  • Temperature
  • Time Factors
  • Zebrafish

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