Multiple sequence-specific transcription factors modulate cytomegalovirus enhancer activity in vitro

P Ghazal; H Lubon; L Hennighausen

Multiple sequence-specific transcription factors modulate cytomegalovirus enhancer activity in vitro

P Ghazal, H Lubon, L Hennighausen

Deanery of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The possibility of DNA-binding proteins interacting in vitro with the polymerase II transcriptional machinery was explored by using a competition assay with individual target sequences for enhancer-binding factors. Transcription factors binding to at least five specific enhancer sequences mediate the activity of the human cytomegalovirus immediate-early 1 gene in vitro. Furthermore, our data suggest that individual DNA-bound enhancer factors can interact with the promoter transcription complex.

Original language	English
Pages (from-to)	1809-11
Number of pages	3
Journal	Molecular and Cellular Biology
Volume	8
Issue number	4
Publication status	Published - 1988

Keywords / Materials (for Non-textual outputs)

Cytomegalovirus
Enhancer Elements, Genetic
Genes, Viral
Kinetics
Plasmids
RNA Polymerase II
Transcription Factors
Transcription, Genetic

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Multiple sequence-specific transcription factors modulate cytomegalovirus enhancer activity in vitro
Copyright © 2013 by the American Society for Microbiology.
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title = "Multiple sequence-specific transcription factors modulate cytomegalovirus enhancer activity in vitro",

abstract = "The possibility of DNA-binding proteins interacting in vitro with the polymerase II transcriptional machinery was explored by using a competition assay with individual target sequences for enhancer-binding factors. Transcription factors binding to at least five specific enhancer sequences mediate the activity of the human cytomegalovirus immediate-early 1 gene in vitro. Furthermore, our data suggest that individual DNA-bound enhancer factors can interact with the promoter transcription complex.",

keywords = "Cytomegalovirus, Enhancer Elements, Genetic, Genes, Viral, Kinetics, Plasmids, RNA Polymerase II, Transcription Factors, Transcription, Genetic",

author = "P Ghazal and H Lubon and L Hennighausen",

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year = "1988",

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journal = "Molecular and Cellular Biology",

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publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Multiple sequence-specific transcription factors modulate cytomegalovirus enhancer activity in vitro

AU - Ghazal, P

AU - Lubon, H

AU - Hennighausen, L

N1 - no DOI

PY - 1988

Y1 - 1988

N2 - The possibility of DNA-binding proteins interacting in vitro with the polymerase II transcriptional machinery was explored by using a competition assay with individual target sequences for enhancer-binding factors. Transcription factors binding to at least five specific enhancer sequences mediate the activity of the human cytomegalovirus immediate-early 1 gene in vitro. Furthermore, our data suggest that individual DNA-bound enhancer factors can interact with the promoter transcription complex.

AB - The possibility of DNA-binding proteins interacting in vitro with the polymerase II transcriptional machinery was explored by using a competition assay with individual target sequences for enhancer-binding factors. Transcription factors binding to at least five specific enhancer sequences mediate the activity of the human cytomegalovirus immediate-early 1 gene in vitro. Furthermore, our data suggest that individual DNA-bound enhancer factors can interact with the promoter transcription complex.

KW - Cytomegalovirus

KW - Enhancer Elements, Genetic

KW - Genes, Viral

KW - Kinetics

KW - Plasmids

KW - RNA Polymerase II

KW - Transcription Factors

KW - Transcription, Genetic

M3 - Article

C2 - 2837656

SN - 0270-7306

VL - 8

SP - 1809

EP - 1811

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

IS - 4

ER -

Multiple sequence-specific transcription factors modulate cytomegalovirus enhancer activity in vitro

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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