Multiplexing for Oxidative Bisulfite Sequencing (oxBS-seq)

Kristina Kirschner, Felix Krueger, Anthony R Green, Tamir Chandra

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

DNA modifications, especially methylation, are known to play a crucial part in many regulatory processes in the cell. Recently, 5-hydroxymethylcytosine (5hmC) was discovered, a DNA modification derived as an intermediate of 5-methylcytosine (5mC) oxidation. Efforts to gain insights into function of this DNA modification are underway and several methods were recently described to assess 5hmC levels using sequencing approaches. Here we integrate adaptation based multiplexing and high-efficiency library prep into the oxidative Bisulfite Sequencing (oxBS-seq) workflow reducing the starting amount and cost per sample to identify 5hmC levels genome-wide.

Original languageEnglish
Title of host publicationDNA Methylation Protocols
PublisherSpringer
Pages665-678
Number of pages14
Volume1708
DOIs
Publication statusE-pub ahead of print - 10 Dec 2017

Publication series

Name Methods in Molecular Biology

Keywords

  • Journal Article

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