Murine DEP-1, a receptor protein tyrosine phosphatase, is expressed in macrophages and is regulated by CSF-1 and LPS

J M Osborne, N den Elzen, A M Lichanska, E O Costelloe, T Yamada, A I Cassady, D A Hume

Research output: Contribution to journalArticlepeer-review


The spectrum of protein tyrosine phosphatases (PTPs) expressed in bone marrow-derived murine macrophages (BMMs) was examined using reverse transcriptase-polymerase chain reaction. Ten different PTP cDNAs were isolated and in this study we focus on mDEP-1, a type III receptor PTP. Three mDEP-1 transcripts were expressed in primary macrophages and macrophage cell lines and were induced during macrophage differentiation of M1 myeloid leukemia cells. A variant mRNA was identified that encodes an alternate carboxyl-terminus and 3' UTR. The expression of mDEP-1 was down-regulated by CSF-1 (macrophage colony-stimulating factor) and up-regulated by bacterial lipopolysaccharide, an important physiological regulator of macrophage function that opposes CSF-1 action. Whole mount in situ hybridization, and immunolocalization of the protein, confirmed that mDEP-1 is expressed by a subset of embryonic macrophages in the liver and mesenchyme. mDEP-1 was also detected in the eye and peripheral nervous system of the developing embryo. Attempts to express mDEP-1 constitutively in the macrophage cell line RAW264 were unsuccessful, with results suggesting that the gene product inhibits cell proliferation.
Original languageEnglish
Pages (from-to)692-701
Number of pages10
JournalJournal of Leukocyte Biology
Issue number5
Publication statusPublished - Nov 1998


  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Bone Marrow Cells
  • Cell Differentiation
  • Cell Division
  • Cell Lineage
  • Cloning, Molecular
  • DNA, Complementary
  • Eye
  • Eye Proteins
  • Fluorescent Antibody Technique, Indirect
  • Gene Expression Regulation, Developmental
  • In Situ Hybridization
  • Lipopolysaccharides
  • Macrophage Colony-Stimulating Factor
  • Macrophages
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Nerve Tissue Proteins
  • Peripheral Nerves
  • Polymerase Chain Reaction
  • Protein Tyrosine Phosphatases
  • RNA, Messenger
  • Receptor-Like Protein Tyrosine Phosphatases, Class 3


Dive into the research topics of 'Murine DEP-1, a receptor protein tyrosine phosphatase, is expressed in macrophages and is regulated by CSF-1 and LPS'. Together they form a unique fingerprint.

Cite this