Murine epithelial cells: isolation and culture

Donald J Davidson, Michael A Gray, Fiona M Kilanowski, Robert Tarran, Scott H Randell, David N Sheppard, Barry E Argent, Julia R Dorin

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We describe an air-liquid interface primary culture method for murine tracheal epithelial cells on semi-permeable membranes, forming polarized epithelia with a high transepithelial resistance, differentiation to ciliated and secretory cells, and physiologically appropriate expression of key genes and ion channels. We also describe the isolation of primary murine nasal epithelial cells for patch-clamp analysis, generating polarised cells with physiologically appropriate distribution and ion channel expression. These methods enable more physiologically relevant analysis of murine airway epithelial cells in vitro and ex vivo, better utilisation of transgenic mouse models of human pulmonary diseases, and have been approved by the European Working Group on CFTR expression.

Original languageEnglish
Pages (from-to)59-62
Number of pages4
JournalJournal of Cystic Fibrosis
Volume3 Suppl 2
DOIs
Publication statusPublished - Aug 2004

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Cells, Cultured
  • Epithelial Cells
  • Histocytological Preparation Techniques
  • Membranes, Artificial
  • Mice
  • Models, Animal
  • Patch-Clamp Techniques
  • Respiratory Mucosa
  • Specimen Handling
  • Trachea

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