Abstract / Description of output
Murine gamma-herpesvirus 68 (MHV-68) is a model for the study of the pathogenesis of gamma-herpesviruses. Epstein-Barr virus (EBV) is a highly related gamma-herpesvirus that causes significant disease in humans. The major membrane antigen gp350 of EBV is a candidate vaccine antigen for protection against EBV-related disease. An MHV-68 glycoprotein, gp150, has significant homology to EBV gp350. We have therefore used the MHV-68 gp150 to model the potential efficacy of EBV gp350 in protecting from virus-associated disease. A recombinant vaccinia virus expressing MHV-68 gp150 was constructed. This recombinant vaccinia virus was used to infect mice via the subcutaneous route. This vaccination resulted in production of MHV-68-neutralising antibodies. Mice were then challenged intra-nasally with MHV-68. MHV-68-associated mononucleosis was virtually abrogated in immunised mice. However, mice did establish MHV-68 latency. The results suggest that gp350 may be effective as an immunogen to prevent EBV-associated infectious mononucleosis in humans that are EBV-seronegative.
| Original language | English |
|---|---|
| Pages (from-to) | 152-7 |
| Number of pages | 6 |
| Journal | Vaccine |
| Volume | 17 |
| Issue number | 2 |
| Publication status | Published - Jan 1999 |
Keywords / Materials (for Non-textual outputs)
- Animals
- Cell Line
- Disease Models, Animal
- Epstein-Barr Virus Infections
- Female
- Gammaherpesvirinae
- Glycoproteins
- Herpesviridae Infections
- Herpesvirus 4, Human
- Humans
- Infectious Mononucleosis
- Mice
- Mice, Inbred BALB C
- Spleen
- Vaccines, Synthetic
- Viral Matrix Proteins
- Viral Proteins
- Viral Vaccines