Murine gamma-herpesvirus 68 glycoprotein 150 protects against virus-induced mononucleosis: a model system for gamma-herpesvirus vaccination

J P Stewart, N Micali, E J Usherwood, L Bonina, A A Nash

Research output: Contribution to journalArticlepeer-review

Abstract

Murine gamma-herpesvirus 68 (MHV-68) is a model for the study of the pathogenesis of gamma-herpesviruses. Epstein-Barr virus (EBV) is a highly related gamma-herpesvirus that causes significant disease in humans. The major membrane antigen gp350 of EBV is a candidate vaccine antigen for protection against EBV-related disease. An MHV-68 glycoprotein, gp150, has significant homology to EBV gp350. We have therefore used the MHV-68 gp150 to model the potential efficacy of EBV gp350 in protecting from virus-associated disease. A recombinant vaccinia virus expressing MHV-68 gp150 was constructed. This recombinant vaccinia virus was used to infect mice via the subcutaneous route. This vaccination resulted in production of MHV-68-neutralising antibodies. Mice were then challenged intra-nasally with MHV-68. MHV-68-associated mononucleosis was virtually abrogated in immunised mice. However, mice did establish MHV-68 latency. The results suggest that gp350 may be effective as an immunogen to prevent EBV-associated infectious mononucleosis in humans that are EBV-seronegative.
Original languageEnglish
Pages (from-to)152-7
Number of pages6
JournalVaccine
Volume17
Issue number2
Publication statusPublished - Jan 1999

Keywords

  • Animals
  • Cell Line
  • Disease Models, Animal
  • Epstein-Barr Virus Infections
  • Female
  • Gammaherpesvirinae
  • Glycoproteins
  • Herpesviridae Infections
  • Herpesvirus 4, Human
  • Humans
  • Infectious Mononucleosis
  • Mice
  • Mice, Inbred BALB C
  • Spleen
  • Vaccines, Synthetic
  • Viral Matrix Proteins
  • Viral Proteins
  • Viral Vaccines

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