Murine gammaherpesvirus 68 establishes a latent infection in mouse B lymphocytes in vivo

N P Sunil-Chandra; S. Efstathiou; Anthony Nash

doi:10.1099/0022-1317-73-12-3275

Murine gammaherpesvirus 68 establishes a latent infection in mouse B lymphocytes in vivo

N P Sunil-Chandra, S. Efstathiou, Anthony Nash

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Murine gammaherpesvirus 68 (MHV-68) is able to persist in spleen cells of infected mice. To determine the cell type harbouring persistent virus, spleen cells from infected animals were separated into immunoglobulin (Ig)-positive (B cell-enriched), Ig-negative (T cell-enriched) and plastic-adherent (macrophage-enriched) fractions. These cells were co-cultivated with permissive BHK-21 cells in an infectious centre assay. The consistent recovery and enrichment of infectious centres in the Ig-positive fraction clearly demonstrates that B cells are a major site of virus persistence/latency. This observation indicates that MHV-68 is biologically similar to Epstein-Barr virus and other members of the B cell lymphotropic gammaherpesvirus 1 subgroup.

Original language	English
Pages (from-to)	3275-9
Number of pages	5
Journal	Journal of General Virology
Volume	73
Issue number	12
DOIs	https://doi.org/10.1099/0022-1317-73-12-3275
Publication status	Published - Dec 1992

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title = "Murine gammaherpesvirus 68 establishes a latent infection in mouse B lymphocytes in vivo",

abstract = "Murine gammaherpesvirus 68 (MHV-68) is able to persist in spleen cells of infected mice. To determine the cell type harbouring persistent virus, spleen cells from infected animals were separated into immunoglobulin (Ig)-positive (B cell-enriched), Ig-negative (T cell-enriched) and plastic-adherent (macrophage-enriched) fractions. These cells were co-cultivated with permissive BHK-21 cells in an infectious centre assay. The consistent recovery and enrichment of infectious centres in the Ig-positive fraction clearly demonstrates that B cells are a major site of virus persistence/latency. This observation indicates that MHV-68 is biologically similar to Epstein-Barr virus and other members of the B cell lymphotropic gammaherpesvirus 1 subgroup.",

author = "Sunil-Chandra, {N P} and S. Efstathiou and Anthony Nash",

year = "1992",

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doi = "10.1099/0022-1317-73-12-3275",

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journal = "Journal of General Virology",

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AU - Sunil-Chandra, N P

AU - Efstathiou, S.

AU - Nash, Anthony

PY - 1992/12

Y1 - 1992/12

N2 - Murine gammaherpesvirus 68 (MHV-68) is able to persist in spleen cells of infected mice. To determine the cell type harbouring persistent virus, spleen cells from infected animals were separated into immunoglobulin (Ig)-positive (B cell-enriched), Ig-negative (T cell-enriched) and plastic-adherent (macrophage-enriched) fractions. These cells were co-cultivated with permissive BHK-21 cells in an infectious centre assay. The consistent recovery and enrichment of infectious centres in the Ig-positive fraction clearly demonstrates that B cells are a major site of virus persistence/latency. This observation indicates that MHV-68 is biologically similar to Epstein-Barr virus and other members of the B cell lymphotropic gammaherpesvirus 1 subgroup.

AB - Murine gammaherpesvirus 68 (MHV-68) is able to persist in spleen cells of infected mice. To determine the cell type harbouring persistent virus, spleen cells from infected animals were separated into immunoglobulin (Ig)-positive (B cell-enriched), Ig-negative (T cell-enriched) and plastic-adherent (macrophage-enriched) fractions. These cells were co-cultivated with permissive BHK-21 cells in an infectious centre assay. The consistent recovery and enrichment of infectious centres in the Ig-positive fraction clearly demonstrates that B cells are a major site of virus persistence/latency. This observation indicates that MHV-68 is biologically similar to Epstein-Barr virus and other members of the B cell lymphotropic gammaherpesvirus 1 subgroup.

U2 - 10.1099/0022-1317-73-12-3275

DO - 10.1099/0022-1317-73-12-3275

M3 - Article

SN - 0022-1317

VL - 73

SP - 3275

EP - 3279

JO - Journal of General Virology

JF - Journal of General Virology

IS - 12

ER -

Murine gammaherpesvirus 68 establishes a latent infection in mouse B lymphocytes in vivo

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