Muscle oxygenation and ATP turnover when blood flow is impaired by vascular disease

GJ Kemp, N Roberts, WE Bimson, A Bakran, SP Frostick

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

In exercising muscle, creatine kinase ensures that mismatch between ATP supply and ATP use results in net phosphocreatine (PCr) splitting. This, inter alia, makes P-31 magnetic resonance spectroscopy a useful tool for studying muscle 'energy metabolism' noninvasively in vivo. We combined this with near-infrared spectroscopy (NIRS) to study ATP synthesis and oxygenation in calf muscle of normal subjects and patients with peripheral vascular disease. Experimental and clinical details and basic data have been published elsewhere (G.J. Kemp et al., Journal of Vascular Surgery 34 (2001), 1103-10); we here propose an analysis of interactions between metabolic 'error signals' and cellular PO2 (estimated from NIRS changes, provisionally assumed to reflect deoxymyoglobin). Post-exercise PCr recovery is monoexponential, and the linear relationship between PCr resynthesis rate (= oxidative ATP synthesis) and the perturbation in PCr (conceptually the simplest error signal) is consistent with negative feedback. In patients the inferred 'mitochondrial capacity' (= oxidative ATP synthesis at 'zero' PCr) is decreased by 53 +/- 6%, leading to reduced oxidative ATP contribution in exercise, because of increased deoxygenation. Increased PCr perturbation partially outweighs cellular hypoxia, but as low cellular PO2 is required for capillary-mitochondrion O-2 diffusion, rate-signal relationships may overstate maximum oxidative ATP synthesis rate.

Original languageEnglish
Pages (from-to)317-334
Number of pages18
JournalSpectroscopy-An international journal
Volume16
Issue number3-4
Publication statusPublished - 2002
Event1st International Conference on Biomedical Spectroscopy - CARDIFF, United Kingdom
Duration: 7 Jul 200210 Jul 2002

Keywords / Materials (for Non-textual outputs)

  • MAGNETIC-RESONANCE SPECTROSCOPY
  • HUMAN SKELETAL-MUSCLE
  • CHRONIC-RENAL-FAILURE
  • MITOCHONDRIAL OXIDATIVE-PHOSPHORYLATION
  • ABSOLUTE METABOLITE CONCENTRATIONS
  • ANAEROBIC ENERGY-PRODUCTION
  • NEAR-INFRARED SPECTROSCOPY
  • P-31 NMR-SPECTROSCOPY
  • HUMAN CALF MUSCLE
  • IN-VIVO

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