MUTATIONAL ANALYSIS OF CONSERVED AMINO-ACID MOTIFS IN ECOKI ADENINE METHYLTRANSFERASE

D T F  DRYDEN; D F  WILLCOCK; N E  MURRAY; David Dryden

MUTATIONAL ANALYSIS OF CONSERVED AMINO-ACID MOTIFS IN ECOKI ADENINE METHYLTRANSFERASE

D T F DRYDEN, D F WILLCOCK, N E MURRAY, David Dryden

School of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The EcoKI methyltransferase (M . EcoKI, MTase) contains the amino acid (aa) sequences AAGTA and NPPF believed to represent the two sequences that are strongly conserved in adenine MTases [Klimasauskas et al., Nucleic Acids Res. 17 (1989) 9823-9831]. We have analysed a mutation in the first sequence that abolishes cofactor binding and enzyme activity, and mutations in the second sequence that reduce or abolish activity without affecting cofactor and DNA binding.

Original language	English
Pages (from-to)	123-124
Number of pages	2
Journal	Gene
Volume	157
Issue number	1-2
Publication status	Published - 19 May 1995

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title = "MUTATIONAL ANALYSIS OF CONSERVED AMINO-ACID MOTIFS IN ECOKI ADENINE METHYLTRANSFERASE",

abstract = "The EcoKI methyltransferase (M . EcoKI, MTase) contains the amino acid (aa) sequences AAGTA and NPPF believed to represent the two sequences that are strongly conserved in adenine MTases [Klimasauskas et al., Nucleic Acids Res. 17 (1989) 9823-9831]. We have analysed a mutation in the first sequence that abolishes cofactor binding and enzyme activity, and mutations in the second sequence that reduce or abolish activity without affecting cofactor and DNA binding.",

author = "DRYDEN, \{D T F\} and WILLCOCK, \{D F\} and MURRAY, \{N E\} and David Dryden",

year = "1995",

month = may,

day = "19",

language = "English",

volume = "157",

pages = "123--124",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

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T1 - MUTATIONAL ANALYSIS OF CONSERVED AMINO-ACID MOTIFS IN ECOKI ADENINE METHYLTRANSFERASE

AU - DRYDEN, D T F

AU - WILLCOCK, D F

AU - MURRAY, N E

AU - Dryden, David

PY - 1995/5/19

Y1 - 1995/5/19

N2 - The EcoKI methyltransferase (M . EcoKI, MTase) contains the amino acid (aa) sequences AAGTA and NPPF believed to represent the two sequences that are strongly conserved in adenine MTases [Klimasauskas et al., Nucleic Acids Res. 17 (1989) 9823-9831]. We have analysed a mutation in the first sequence that abolishes cofactor binding and enzyme activity, and mutations in the second sequence that reduce or abolish activity without affecting cofactor and DNA binding.

AB - The EcoKI methyltransferase (M . EcoKI, MTase) contains the amino acid (aa) sequences AAGTA and NPPF believed to represent the two sequences that are strongly conserved in adenine MTases [Klimasauskas et al., Nucleic Acids Res. 17 (1989) 9823-9831]. We have analysed a mutation in the first sequence that abolishes cofactor binding and enzyme activity, and mutations in the second sequence that reduce or abolish activity without affecting cofactor and DNA binding.

M3 - Article

SN - 0378-1119

VL - 157

SP - 123

EP - 124

JO - Gene

JF - Gene

IS - 1-2

ER -

MUTATIONAL ANALYSIS OF CONSERVED AMINO-ACID MOTIFS IN ECOKI ADENINE METHYLTRANSFERASE

Abstract

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