Mycoplasma species in cats with lower airway disease: improved detection and species identification using a polymerase chain reaction assay

Nicki Reed, Kerry Simpson, Roger Ayling, Robin Nicholas, Danielle Gunn-Moore

Research output: Contribution to journalArticlepeer-review

Abstract

There is some evidence that Mycoplasma species may be associated with lower airway disease in cats. Retrospective and prospective studies were carried out on a total population of 76 cats but failed to identify any cases of Mycoplasma species infection by bacterial culture alone. The overall prevalence of bacterial infection (15.8%) was also lower than that identified in previous studies. When a molecular detection technique, the PCR-DGGE, was employed the prevalence of Mycoplasma species detected was 15.4%, with M felis, M gateae and M feliminutum species identified, although the significance of these Mycoplasma species in feline lower airway disease remains in question. However, the PCR-DGGE technique allowed species identification and indicated the presence of M feliminutum, a species not previously isolated from the lower airways of cats.
Original languageEnglish
Pages (from-to)833-840
JournalJournal of Feline Medicine and Surgery
Volume14
Issue number12
Early online date22 Jun 2012
DOIs
Publication statusPublished - 2012

Keywords

  • animals
  • Cat Diseases
  • CATS
  • female
  • male
  • MYCOPLASMA
  • Mycoplasma Infections
  • Polymerase Chain Reaction
  • Prospective Studies
  • Respiratory Tract Diseases
  • Retrospective Studies
  • Sensitivity and Specificity

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