Naf1p is a box H/ACA snoRNP assembly factor

A Fatica, M Dlakic, D Tollervey

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs) contain four essential proteins, Cbf5p, Gar1p, Nhp2p, and Nop10p, each of which, with the exception of Gar1p, is required for box H/ACA snoRNA accumulation. Database searches identified a novel essential protein, which we termed Naf1p, with a region of homology to the RNA-binding domain of Gar1p and other features in common with hnRNP-like proteins. Naf1p is localized to the nucleus and is not a stable component of the H/ACA snoRNPs, but it is required for the accumulation of all box H/ACA snoRNAs. This requirement is not at the level of snoRNA transcription initiation or termination. Naf1p shows in vitro RNA-binding activity and also binds directly to Cbf5p and Nhp2p. Naf1p was shown to bind to the CTD in vivo in a two-hybrid assay, and the phosphorylated CTD, but not the nonphosphorylated CTD, was shown to precipitate tagged Naf1p from a cell lysate. We propose that Naf1p is recruited to the CTD of RNA polymerase 11 and binds to nascent box H/ACA snoRNAs promoting snoRNP assembly.

Original languageEnglish
Pages (from-to)1502-1514
Number of pages13
JournalRNA
Volume8
Issue number12
Publication statusPublished - Dec 2002

Keywords / Materials (for Non-textual outputs)

  • ACA snoRNPs
  • box H
  • snoRNP assembly

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