Nanopore detection of single-molecule binding within a metallosupramolecular cage

Stefan Borsley, James Cooper, Paul Lusby, Scott Cockroft

Research output: Contribution to journalArticlepeer-review

Abstract

Guest encapsulation is a fundamental property of coordination cages. However, there is a paucity of methods capable of quantifying the dynamics of guest binding processes. Here, we demonstrate nanopore detection of single-molecule binding within metallosupramolecular cages. Real-time monitoring of the ion current flowing through a transmembrane -hemolysin nanopore resolved the binding of different guests to both cage enantiomers. This enabled the single-molecule kinetics of guest binding to be quantified, while the ordering and durations of events were consistent with a guestexchange
mechanism that does not involve ligand dissociation. In addition to providing a new approach for single-molecule interrogation of dynamic supramolecular processes, we also established that cage complexes which are too large to enter the nanopore can be exploited for detecting small molecules, thus constituting a new class of molecular adapter.
Original languageEnglish
JournalChemistry - A European Journal
Early online date15 Feb 2018
DOIs
Publication statusE-pub ahead of print - 15 Feb 2018

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