Nanoscale structure of amyloid-β plaques in Alzheimer’s disease

Marta Querol-Vilaseca, Marti Colom-Cadena, Jordi Pegueroles, Raul Nunez-Llaves, Joan Luque-Cabecerans, Laia Munoz-Llahuna, Jordi Andilla, Olivia Belbin, Tara Spires-Jones, Ellen Gelpi, Jordi Clarimon, Pablo Loza-Alvarez,, Juan Fortea, Alberto Lleo

Research output: Contribution to journalArticlepeer-review

Abstract

Soluble amyloid-β (Aβ) is considered to be a critical component in the pathogenesis of Alzheimer’s disease (AD). Evidence suggests that these non-fibrillar Aβ assemblies are implicated in synaptic dysfunction, neurodegeneration and cell death. However, characterization of these species comes mainly from studies in cellular or animal models, and there is little data in intact human samples due to the lack of adequate optical microscopic resolution to study these small structures. Here, to achieve super-resolution in all three dimensions, we applied Array Tomography (AT) and Stimulated Emission Depletion microscopy (STED), to characterize in postmortem human brain tissue non-fibrillar Aβ structures in amyloid plaques of cases with autosomal dominant and sporadic AD. Ultrathin sections scanned with super-resolution STED microscopy allowed the detection of small Aβ structures of the order of 100 nm. We reconstructed a whole human amyloid plaque and established that plaques are formed by a dense core of higher order Aβ species (~0.022 µm3) and a peripheral halo of smaller Aβ structures (~0.003 µm3). This work highlights the potential of AT-STED for human neuropathological studies.
Original languageEnglish
Article number5181
JournalScientific Reports
Volume9
Early online date26 Mar 2019
DOIs
Publication statusE-pub ahead of print - 26 Mar 2019

Fingerprint

Dive into the research topics of 'Nanoscale structure of amyloid-β plaques in Alzheimer’s disease'. Together they form a unique fingerprint.

Cite this