Natural variation reveals that intracellular distribution of ELF3 protein is associated with function in the circadian clock

Muhammad Usman Anwer, Eleni Boikoglou, Eva Herrero, Marc Hallstein, Amanda Melaragno Davis, Geo Velikkakam James, Ferenc Nagy, Seth Jon Davis

Research output: Contribution to journalArticlepeer-review

Abstract

Natural selection of variants within the Arabidopsis thaliana circadian clock can be attributed to adaptation to varying environments. To define a basis for such variation, we examined clock speed in a reporter-modified Bay-0 x Shakdara recombinant inbred line and localized heritable variation. Extensive variation led us to identify EARLY FLOWERING3 (ELF3) as a major quantitative trait locus (QTL). The causal nucleotide polymorphism caused a short-period phenotype under light and severely dampened rhythm generation in darkness, and entrainment alterations resulted. We found that ELF3-Sha protein failed to properly localize to the nucleus, and its ability to accumulate in darkness was compromised. Evidence was provided that the ELF3-Sha allele originated in Central Asia. Collectively, we showed that ELF3 protein plays a vital role in defining its light-repressor action in the circadian clock and that its functional abilities are largely dependent on its cellular localization.

Original languageEnglish
JournaleLIFE
Volume3
DOIs
Publication statusPublished - 27 May 2014

Keywords

  • Alleles
  • Arabidopsis
  • Arabidopsis Proteins
  • Cell Nucleus
  • Chromosome Mapping
  • Circadian Clocks
  • Flowers
  • Geography
  • Light
  • Microscopy, Confocal
  • Mutation
  • Phenotype
  • Phylogeny
  • Promoter Regions, Genetic
  • Quantitative Trait Loci
  • Transcription Factors
  • Transgenes

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